Mouse, Human 交差が予測される動物種:
Chimpanzee, Rhesus monkey, Gorilla, Orangutan
Synthetic peptide, corresponding to a region within C terminal amino acids 770-820 of Human Smek1 (NP_115949.3)
Human Breast Carcinoma, Human Colon Carcinoma, Human Lung Adenocarcinoma, Human Non-Small Cell Lung Cancer, Human Placenta, Human Prostate Carcinoma, Human Stomach Adenocarcinoma, Human Testicular Seminoma, Mouse Renal Cell Carcinoma, Mouse Teratoma
保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
バッファーPreservative: 0.09% Sodium Azide
Constituents: 0.1% BSA, Tris buffered saline
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
追加情報IHC-P: 1/100 - 1/500. Epitope exposure is recommended. Epitope exposure with Tris-EDTA pH9.0 buffer will enhance staining.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
機能Regulatory subunit of serine/threonine-protein phosphatase 4. May regulate the activity of PPP4C at centrosomal microtubule organizing centers. The PPP4C-PPP4R2-PPP4R3A PP4 complex specifically dephosphorylates H2AFX phosphorylated on 'Ser-140' (gamma-H2AFX) generated during DNA replication and required for DNA DSB repair.
配列類似性Belongs to the SMEK family. Contains 1 WH1 domain.
細胞内局在Cytoplasm. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Nucleus. In interphase localized in the cytoplasm and in the nucleus (with higher levels). During metaphase located in pericentriolar regions.
ab91618, at 1/250 dilution, staining Smek1 in formalin-fixed paraffin-embedded Human breast carcinoma tissue by Immunohistochemistry using Red-fluorescent goat anti-rabbit IgG highly cross-adsorbed Antibody Hilyte PlusTM 555 secondary antibody at a dilution of 1/100.
Anti-Smek1 antibody (ab91618) 使用論文
has not yet been referenced specifically in any publications.