製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to SMCR7
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Rat, Human
  • 免疫原

    Recombinant fragment, corresponding to a region within amino acids 299-441 of Human SMCR7 (AAH14973).

  • ポジティブ・コントロール
    • 293T, A431, Jurkat and Raji whole cell lysates.

法規制情報

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファー
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 20% Glycerol, 0.1M Tris, 0.1M Glycine, pH 7.0
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab101350 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/3000. Predicted molecular weight: 49 kDa.

ターゲット情報

  • 機能
    Mitochondrial outer membrane protein which regulates mitochondrial fission. Promotes the recruitment and association of the fission mediator dynamin-related protein 1 (DNM1L) to the mitochondrial surface independently of the mitochondrial fission FIS1 and MFF proteins. Regulates DNM1L GTPase activity.
  • 組織特異性
    Expressed in all tissues tested with highest expression in heart and skeletal muscle.
  • 配列類似性
    Belongs to the MID49/MID51 family.
  • 細胞内局在
    Mitochondrion outer membrane. Colocalizes with DNM1L at mitochondrial membrane. Forms foci and rings around mitochondria.
  • Information by UniProt
  • 参照データベース
  • 別名
    • MGC23130 antibody
    • MID49 antibody
    • MID49_HUMAN antibody
    • MIEF2 antibody
    • Mitochondrial dynamic protein MID49 antibody
    • Mitochondrial dynamic protein of 49 kDa antibody
    • Mitochondrial dynamics protein MID49 antibody
    • Mitochondrial dynamics protein of 49 kDa antibody
    • Mitochondrial elongation factor 2 antibody
    • Smith Magenis syndrome chromosome region candidate 7 antibody
    • Smith-Magenis syndrome chromosomal region candidate gene 7 protein antibody
    see all

画像

  • Anti-SMCR7 antibody (ab101350) at 1/500 dilution + Raji whole cell lysate at 30 µg

    Predicted band size : 49 kDa

プロトコール

参考文献

ab101350 has not yet been referenced specifically in any publications.

レビューと Q&A

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Loading amount
30 µg
Gel Running Conditions
Reduced Denaturing (4-12)
Sample
Rat Cell lysate - whole cell (Primary Neurons)
Specification
Primary Neurons
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

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投稿 Sep 05 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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