製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to SMC6L1
  • アプリケーション
    適用あり: IHC-Pmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Chimpanzee
  • 免疫原

    Synthetic peptide corresponding to a region between residue 1050 and the C-terminus (residue 1091) of Human SMC6L1 (Swiss-Prot Q96SB8)

  • ポジティブ・コントロール
    • Human ovarian and breast tumor tissues.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab86103 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P
  • 追加情報
    IHC-P: 1/100 - 1/500. Epitope exposure is recommended. Epitope exposure with citrate buffer will enhance staining.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能
      Core component of the SMC5-SMC6 complex, a complex involved in DNA double-strand breaks by homologous recombination. The complex may promote sister chromatid homologous recombination by recruiting the SMC1-SMC3 cohesin complex to double-strand breaks. The complex is required for telomere maintenance via recombination in ALT (alternative lengthening of telomeres) cell lines and mediates sumoylation of shelterin complex (telosome) components which is proposed to lead to shelterin complex disassembly in ALT-associated PML bodies (APBs). Required for recruitment of telomeres to PML nuclear bodies.
    • 組織特異性
      Widely expressed. Overexpressed in testis.
    • 配列類似性
      Belongs to the SMC family. SMC6 subfamily.
    • ドメイン
      The flexible hinge domain, which separates the large intramolecular coiled coil regions, allows the heterotypic interaction with the corresponding domain of SMC5, forming a V-shaped heterodimer.
    • 翻訳後修飾
      Sumoylated by NSMCE2/MMS21.
      Ubiquitinated.
    • 細胞内局在
      Nucleus. Chromosome. Chromosome > telomere. Associates with chromatin. Localizes to PML nuclear bodies in ALT cell lines.
    • Information by UniProt
    • 参照データベース
    • 別名
      • FLJ22116 antibody
      • FLJ35534 antibody
      • hSMC6 antibody
      • SMC 6 antibody
      • SMC protein 6 antibody
      • SMC-6 antibody
      • SMC6 antibody
      • SMC6 protein antibody
      • SMC6 structural maintenance of chromosomes 6 like 1 antibody
      • SMC6_HUMAN antibody
      • Smc6l1 antibody
      • Structural maintenance of chromosomes 6 antibody
      • Structural maintenance of chromosomes 6 like 1 antibody
      • Structural maintenance of chromosomes protein 6 antibody
      see all

    Anti-SMC6L1 antibody 画像

    • ab86103, at 1/250 dilution, staining SMC6L1 in formalin-fixed, paraffin-embedded human ovarian tumor tissue by Immunohistochemistry using DAB staining.
    • ab86103, at 1/250 dilution, staining SMC6L1 in formalin-fixed, paraffin-embedded human breast tumor tissue by Immunohistochemistry using DAB staining.

    プロトコール

    Anti-SMC6L1 antibody (ab86103) 使用論文

    ab86103 has not yet been referenced specifically in any publications.

    Product Wall

    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (HeLa)
    Specification
    HeLa
    Permeabilization
    Yes - 0.5% Triton X100 in PBS
    Fixative
    Paraformaldehyde
    Username

    Dr. Kirk Mcmanus

    Verified customer

    投稿 Nov 15 2013

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    登録