製品の概要

  • 製品名Anti-SMC6L1 antibody
    SMC6L1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to SMC6L1
  • アプリケーション適用あり: IHC-P, WB, IP, ICC/IFmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Chimpanzee
  • 免疫原

    Synthetic peptide:

    PQSMSSLPSSKLIRILRMSDPERGQTTLPFR

    , corresponding to C terminal amino acids 1050-1091 of Human SMC6L1.

  • ポジティブ・コントロール
    • Whole cell lysate from lymphoblastoid cells (TK6). Whole cell lysate from HeLa and 293T cells.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab18039 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB 1/5000 - 1/25000. Detects a band of approximately 140 kDa (predicted molecular weight: 126 kDa).
IP Use at 5-10 µg/mg of lysate.
ICC/IF 1/200.

ターゲット情報

  • 機能Core component of the SMC5-SMC6 complex, a complex involved in DNA double-strand breaks by homologous recombination. The complex may promote sister chromatid homologous recombination by recruiting the SMC1-SMC3 cohesin complex to double-strand breaks. The complex is required for telomere maintenance via recombination in ALT (alternative lengthening of telomeres) cell lines and mediates sumoylation of shelterin complex (telosome) components which is proposed to lead to shelterin complex disassembly in ALT-associated PML bodies (APBs). Required for recruitment of telomeres to PML nuclear bodies.
  • 組織特異性Widely expressed. Overexpressed in testis.
  • 配列類似性Belongs to the SMC family. SMC6 subfamily.
  • ドメインThe flexible hinge domain, which separates the large intramolecular coiled coil regions, allows the heterotypic interaction with the corresponding domain of SMC5, forming a V-shaped heterodimer.
  • 翻訳後修飾Sumoylated by NSMCE2/MMS21.
    Ubiquitinated.
  • 細胞内局在Nucleus. Chromosome. Chromosome > telomere. Associates with chromatin. Localizes to PML nuclear bodies in ALT cell lines.
  • Information by UniProt
  • 参照データベース
  • 別名
    • FLJ22116 antibody
    • FLJ35534 antibody
    • hSMC6 antibody
    • SMC 6 antibody
    • SMC protein 6 antibody
    • SMC-6 antibody
    • SMC6 antibody
    • SMC6 protein antibody
    • SMC6 structural maintenance of chromosomes 6 like 1 antibody
    • SMC6_HUMAN antibody
    • Smc6l1 antibody
    • Structural maintenance of chromosomes 6 antibody
    • Structural maintenance of chromosomes 6 like 1 antibody
    • Structural maintenance of chromosomes protein 6 antibody
    see all

Anti-SMC6L1 antibody 画像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma tissue labelling SMC6L1 with ab18039 at 1/1000 (1µg/ml). Detection: DAB.


  • Predicted band size : 126 kDa

    Detection of Human SMC6 by Western Blot and Immunoprecipitation. Samples:

    A) Whole cell lysate (WCL; 10, 20 and 50 µg) from lymphoblastoid cells (TK6).

    B) Whole cell lysate (500 µg) from HeLa and 293T cells.

    Antibody: Affinity purified rabbit anti-SMC6 antibody used at 0.1 µg/ml for WB and at 5 µg/0.5 mg lysate for IP.

    Detection: Chemiluminescence with exposure times of less than 2 minutes.

    Detection of Human SMC6 by Western Blot and Immunoprecipitation. Samples:

    A) Whole cell lysate (WCL; 10, 20 and 50 µg) from lymphoblastoid cells (TK6).

    B) Whole cell lysate (500 <>

  • ab18039 (1/200) staining SMC6L in HeLa cells (green). Cells were fixed in methanol, permeabilised with 0.5% Triton X100/PBS and counterstained with DAPI in order to highlight the nucleus (red). Please refer to abreview for further experimental details.

    See Abreview

  • IHC image of ab18039 staining in human normal tonsil formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH9, epitope retrieval solution 2) for 20 mins. The section was then incubated with ab18039, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-SMC6L1 antibody (ab18039) 使用論文

This product has been referenced in:
  • Verver DE  et al. The SMC5/6 complex is involved in crucial processes during human spermatogenesis. Biol Reprod 91:22 (2014). WB ; Human . Read more (PubMed: 24855106) »
  • Hopkins J  et al. Meiosis-specific cohesin component, Stag3 is essential for maintaining centromere chromatid cohesion, and required for DNA repair and synapsis between homologous chromosomes. PLoS Genet 10:e1004413 (2014). ICC/IF ; Mouse . Read more (PubMed: 24992337) »

See all 4 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HeLa)
Specification HeLa
Fixative Methanol
Permeabilization Yes - 0.5% TritonX100 in PBS
Username

Dr. Kirk McManus

Verified customer

投稿 Nov 05 2007

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"