製品の概要

  • 製品名Anti-SMC4 antibody
    SMC4 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to SMC4
  • アプリケーション適用あり: WB, ICC/IFmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Rabbit, Horse, Guinea pig, Dog, Chimpanzee, Ferret, Rhesus monkey, Gorilla, Elephant
  • 免疫原

    Synthetic peptide, which represented a portion of human structural maintenance of chromosomes 4 encoded within exons 11 and 12.

  • ポジティブ・コントロール
    • SMC4 transfected 239T cells.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab17957 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000 - 1/10000. Detects a band of approximately 147 kDa (predicted molecular weight: 147 kDa).
ICC/IF 1/500 - 1/2000.

ターゲット情報

  • 機能Central component of the condensin complex, a complex required for conversion of interphase chromatin into mitotic-like condense chromosomes. The condensin complex probably introduces positive supercoils into relaxed DNA in the presence of type I topoisomerases and converts nicked DNA into positive knotted forms in the presence of type II topoisomerases.
  • 組織特異性Widely expressed. Higher expression in testis, colon, thymus.
  • 配列類似性Belongs to the SMC family. SMC4 subfamily.
  • ドメインThe hinge domain, which separates the large intramolecular coiled coil regions, allows the heterodimerization with SMC2, forming a V-shaped heterodimer.
  • 細胞内局在Nucleus. Cytoplasm. Chromosome. In interphase cells, the majority of the condensin complex is found in the cytoplasm, while a minority of the complex is associated with chromatin. A subpopulation of the complex however remains associated with chromosome foci in interphase cells. During mitosis, most of the condensin complex is associated with the chromatin. At the onset of prophase, the regulatory subunits of the complex are phosphorylated by CDC2, leading to condensin's association with chromosome arms and to chromosome condensation. Dissociation from chromosomes is observed in late telophase.
  • Information by UniProt
  • 参照データベース
  • 別名
    • CAP C antibody
    • CAPC antibody
    • Chromosome associated polypeptide C antibody
    • Chromosome-associated polypeptide C antibody
    • hCAP C antibody
    • hCAP-C antibody
    • SMC 4 antibody
    • SMC protein 4 antibody
    • SMC-4 antibody
    • SMC4 antibody
    • SMC4_HUMAN antibody
    • SMC4L1 antibody
    • Structural maintenance of chromosomes 4 antibody
    • Structural maintenance of chromosomes 4 like 1 antibody
    • Structural maintenance of chromosomes protein 4 antibody
    • XCAP C homolog antibody
    • XCAP-C homolog antibody
    see all

Anti-SMC4 antibody 画像

  • Immunocytochemistry/Immunofluorescence analysis of HeLa cells labelling SMC4 with ab17957 at 5µg/ml. Cells were fixed with methanol/actetone and blocked with 3% BSA in PBS. A Rhodamine-conjugated anti-rabbit IgG was used as the secondary antibody. Cells were counterstained with DAPI.


  • Predicted band size : 147 kDa

    Samples: Whole cell lysate (90 µg) from 293T cells (lane 1), SMC4 transfected 239T cells (lane 2) or normal human lymphoblasts (lane 3) separated on an 8% SDS-PAGE gel. Antibodies: ab17957 used at a dilution of 1/5,000 and goat anti-rabbit HRP used at a dilution of 1:20,000. Detection: Chemiluminescence with 5 second exposure.

    Samples: Whole cell lysate (90 µg) from 293T cells (lane 1), SMC4 transfected 239T cells (lane 2) or normal human lymphoblasts (lane 3) separated on an 8% SDS-PAGE gel. Antibodies: ab17957 used at a dilution of 1/5,000 and goat anti-rabbit HRP used at a dilution of 1:20,000. Detection: Chemiluminescence with 5 second exposure.

Anti-SMC4 antibody (ab17957) 使用論文

ab17957 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HeLa)
Specification HeLa
Fixative Paraformaldehyde
Permeabilization Yes - 0.5% TritonX100 in PBS
Username

Dr. Kirk McManus

Verified customer

投稿 Nov 02 2007

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"