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Rabbits were immunized with a phosphorylated synthetic peptide, which represented a portion of human Structural Maintenance of Chromosomes-3 (LocusLink ID 9126)around Serine 383, conjugated to KLH. After multiple immunizations in Freund's adjuvant, serum was collected.
Our Abpromise guarantee covers the use of ab1277 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Western Blot of Immunoprecipitation products and also neat Hela nuclear cell extract +/- IR treatment:
ab1277 has not yet been referenced specifically in any publications.