製品の概要

  • 製品名Anti-SMC3 antibody - ChIP Grade
    SMC3 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to SMC3 - ChIP Grade
  • アプリケーション適用あり: ICC, IP, WB, ChIP, IHC-Pmore details
  • 種交差性
    交差種: Mouse, Human, Xenopus laevis
    交差が予測される動物種: Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Platypus (Ornithorhynchus anatinus)
  • 免疫原

    Synthetic peptide (Human) conjugated to KLH - which represents a portion within the last 100 amino acids of the human Structural Maintenance of Chromosomes-3 (GenBank PID 4885399), conjugated toKLH.

  • ポジティブ・コントロール
    • HeLa nuclear or whole cell extract

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab9263 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC 1/200 - 1/800.
IP Use at 2-10 µg/mg of lysate.
WB 1/1000 - 1/10000. Detects a band of approximately 140 kDa (predicted molecular weight: 141.5 kDa).
ChIP Use at an assay dependent concentration. PubMed: 20966046
IHC-P 1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

ターゲット情報

  • 機能Central component of cohesin, a complex required for chromosome cohesion during the cell cycle. The cohesin complex may form a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. Cohesion is coupled to DNA replication and is involved in DNA repair. The cohesin complex plays also an important role in spindle pole assembly during mitosis and in chromosomes movement.
  • 関連疾患Defects in SMC3 are the cause of Cornelia de Lange syndrome type 3 (CDLS3) [MIM:610759]. CDLS is a dominantly inherited multisystem developmental disorder characterized by growth and cognitive retardation, abnormalities of the upper limbs, gastroesophageal dysfunction, cardiac, ophthalmologic and genitourinary anomalies, hirsutism, and characteristic facial features. CDSL3 is a mild form with absence of major structural anomalies typically associated with CDLS. The phenotype in some instances approaches that of apparently non-syndromic mental retardation.
  • 配列類似性Belongs to the SMC family. SMC3 subfamily.
  • ドメインThe flexible hinge domain, which separates the large intramolecular coiled coil regions, allows the heterotypic interaction with the corresponding domain of SMC1A or SMC1B, forming a V-shaped heterodimer. The two heads of the heterodimer are then connected by different ends of the cleavable RAD21 protein, forming a ring structure.
  • 翻訳後修飾Phosphorylated upon DNA damage, probably by ATM or ATR.
    Acetylation at Lys-105 and Lys-106 by ESCO1 is important for genome stability and S phase sister chromatid cohesion. Regulated by DSCC1, it is required for processive DNA synthesis, coupling sister chromatid cohesion establishment during S phase to DNA replication.
  • 細胞内局在Nucleus. Chromosome. Chromosome > centromere. Associates with chromatin. Before prophase it is scattered along chromosome arms. During prophase, most of cohesin complexes dissociate from chromatin probably because of phosphorylation by PLK, except at centromeres, where cohesin complexes remain. At anaphase, the RAD21 subunit of the cohesin complex is cleaved, leading to the dissociation of the complex from chromosomes, allowing chromosome separation.
  • Information by UniProt
  • 参照データベース
  • 別名
    • BAM antibody
    • Bamacan antibody
    • Basement membrane associated chondroitin proteoglycan antibody
    • Basement membrane-associated chondroitin proteoglycan antibody
    • BMH antibody
    • CDLS3 antibody
    • chondroitin sulfate proteoglycan 6 (bamacan) antibody
    • Chondroitin sulfate proteoglycan 6 antibody
    • Chromosome associated polypeptide antibody
    • Chromosome-associated polypeptide antibody
    • CSPG 6 antibody
    • CSPG6 antibody
    • hCAP antibody
    • Human chromosome associated polypeptide antibody
    • im:7142991 antibody
    • SMC 3 antibody
    • SMC protein 3 antibody
    • SMC-3 antibody
    • smc3 antibody
    • SMC3_HUMAN antibody
    • SMC3L1 antibody
    • Structural maintenance of chromosome 3 antibody
    • Structural maintenance of chromosomes 3 antibody
    • Structural maintenance of chromosomes protein 3 antibody
    • u:fb22e01 antibody
    • wu:fc30d07 antibody
    see all

Anti-SMC3 antibody - ChIP Grade 画像

  • ab9263 (4µg/ml) staining SMC3 in human colon using an automated system (DAKO Autostainer Plus). Using this protocol there is strong nuclear staining.
    Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffer citrate pH 6.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
  • All lanes : Anti-SMC3 antibody - ChIP Grade (ab9263) at 1 µg/ml

    Lane 1 : Human placenta tissue lysate - total protein (ab29745)
    Lane 2 : K562 (Human erythromyeloblastoid leukemia cell line) Whole Cell Lysate
    Lane 3 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Predicted band size : 141.5 kDa
    Observed band size : 145 kDa (why is the actual band size different from the predicted?)
  • SMC3 signals (green) in a human spermatocyte at the zygotene-pachytene transition stage. Wider signals correspond to synapsed areas, whereas narrower signals show non-synapsed regions.

    This image was kindly supplied as part of the review submitted by Maria Oliver-Bonet.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human testicular seminoma tissue labelling SMC3 with ab9263 at 1/1000 (1µg/ml). Detection: DAB.
  • ab9263 Immunoprecipitate in human HeLa cytosolic and nuclear extracts. 500µg of cell lysate incubated with primary antibody (1/100) and matrix (Protein A) in 20mM HEPES ph 7.8, 100mM KAc for 4 hours at 4°C. For Western blotting ab9263 was used at a dilution at 1/1000.

    See Abreview

  • Interphase HeLa cells stained with ab9263 (1/500). The antibody gave predominantly nuclear staining in all interphase cells investigated. ab9263 staining is shown in green. The cells were counterstained with DAPI (red).

Anti-SMC3 antibody - ChIP Grade (ab9263) 使用論文

This product has been referenced in:
  • Kracker S  et al. An inherited immunoglobulin class-switch recombination deficiency associated with a defect in the INO80 chromatin remodeling complex. J Allergy Clin Immunol 135:998-1007.e6 (2015). Read more (PubMed: 25312759) »
  • Qiu Z  et al. Functional interactions between NURF and Ctcf regulate gene expression. Mol Cell Biol 35:224-37 (2015). Read more (PubMed: 25348714) »

See all 21 Publications for this product

Product Wall


I have performed an alignment of the sequence provided in the word document with the sequence used for ab9263. At the site recognized by ab9263, the human and Schistosoma mansoni (blood fluke) sequences are too divergent to expect ab9263 to bind. ...

Read More



Unfortunately, at the site recognized by ab9263, the human and Schistosoma mansoni sequences are too divergent to expect ab9263 to bind.

However, Anti-SMC3 antibody's (ab74582) immunogen shows a 90% sequence homology with the above ...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (Hela)
Loading amount 50 µg
Specification Hela
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Abcam user community

Verified customer

投稿 Aug 01 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunoprecipitation
Sample Human Cell lysate - whole cell (HeLa cytosolic and nuclear extracts)
Total protein in input 500 µg
Specification HeLa cytosolic and nuclear extracts
Immuno-precipitation step Protein A
Username

Abcam user community

Verified customer

投稿 Nov 03 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HeLa cytosolic and nuclear extracts)
Loading amount 50 µg
Specification HeLa cytosolic and nuclear extracts
Gel Running Conditions Reduced Denaturing (8% gel)
Blocking step Milk as blocking agent for 14 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Abcam user community

Verified customer

投稿 Nov 02 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry
Sample Human Cell (HeLa)
Specification HeLa
Fixative Paraformaldehyde
Username

Dr. Kirk McManus

Verified customer

投稿 Dec 07 2005

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Xenopus laevis Purified protein (Chromatin isolated from Xenopus egg extract)
Specification Chromatin isolated from Xenopus egg extract
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5
Username

Dr. Alan Score

Verified customer

投稿 Nov 28 2005

Ab3600 - Rabbit polyclonal to Argonaute 4 - has not been discontinued; it is currently in stock in our office in the UK. If you have any more questions, please contact me again.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"