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Immunogen was a synthetic peptide, which represented a portion of human structural maintenance of chromosomes 2 encoded within exon 4 (LocusLink ID 10592).
Our Abpromise guarantee covers the use of ab10399 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/5000 - 1/20000. Detects a band of approximately 150 kDa (predicted molecular weight: 143 kDa).|
|IHC-P||Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|