Segregation of mitotic chromosomes like 1 antibody
SMC 1 antibody
SMC protein 1B antibody
SMC1alpha protein antibody
SMC1beta protein antibody
Structural maintenance of chromosome 1 like 1 protein antibody
Structural maintenance of chromosome 1 like 2 protein antibody
Structural maintenance of chromosomes 1A antibody
Structural maintenance of chromosomes 1B antibody
Structural maintenance of chromosomes protein 1B antibody
Western blot - Anti-SMC1 antibody (ab21583)
All lanes : Anti-SMC1 antibody (ab21583) at 1 µg/ml
Lane 1 : 20ug HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate Lane 2 :Jurkat whole cell lysate (ab7899) at 20 µg Lane 3 : 20ug HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate with Human SMC1 peptide (ab23863) at 1 µg/ml Lane 4 :Jurkat whole cell lysate (ab7899) at 20 µg with Human SMC1 peptide (ab23863) at 1 µg/ml
Secondary All lanes : Goat polyclonal to Rabbit IgG (Alexa Fluor® 680) (ab28446) at 1/10000 dilution
SMC1 was immunoprecipitated using 0.5mg Hela whole cell extract, 5µg of Rabbit polyclonal to SMC1 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-). The antibody was incubated under agitation with Protein G beads for 10min, Hela whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation. Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab21583. Secondary: Goat polyclonal to mouse IgG light chain specific (HRP) at 1/5000 dilution. Band: 150kDa: SMC1; Non specific - 41 and 42kDa: We are unsure as to the identity of this extra band.
ICC/IF image of ab21583 stained human HeLa cells. The cells were methanol fixed (5 min) and incubated with the antibody (ab21583, 1µg/ml) for 1h at room temperature. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Image-iTTM FX Signal Enhancer was used as the primary blocking agent, 5% BSA (in TBS-T) was used for all other blocking steps. DAPI was used to stain the cell nuclei (blue). Alexa Fluor® 594 WGA was used to label plasma membranes (red).
Panel A shows localisation of ab21583 to the nuclei, Panel B has the Alexa Fluor® 488 channel removed for comparison.
Immunocytochemistry/ Immunofluorescence - Anti-SMC1 antibody (ab21583)This image is courtesy of an Abreview submitted by Dr Kirk McManus
ab21583 staining SMC1 in assynchonous HeLa cells (green). Cells were paraformaldehyde-fixed (4% - 10min) and counterstained with DAPI (red). Secondary antibody: Goat anti-Rabbit conjugated to Cy3 ®. Please refer to Abreview for further details.
IHC image of SMC1 staining in human skin FFPE section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab21583, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Parenti I et al. Overall and allele-specific expression of the SMC1A gene in female Cornelia de Lange syndrome patients and healthy controls. Epigenetics9:N/A (2014).
Read more (PubMed: 24756084) »
Hopkins J et al. Meiosis-specific cohesin component, Stag3 is essential for maintaining centromere chromatid cohesion, and required for DNA repair and synapsis between homologous chromosomes. PLoS Genet10:e1004413 (2014).
Read more (PubMed: 24992337) »