製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to SMARCD1
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    Three synthetic peptides (human) conjugated to KLH

  • ポジティブ・コントロール
    • An induced culture of E. coli

法規制情報

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab86029 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000. Detects a band of approximately 72 kDa (predicted molecular weight: 58 kDa). Tag is 14 kDa.

ターゲット情報

  • 機能
    Involved in chromatin remodeling. Belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). During neural development a switch from a stem/progenitor to a post-mitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to post-mitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth (By similarity). Has a strong influence on the Vitamin D-mediated transcriptional activity from an enhancer Vitamin D receptor element (VDRE). May be a link between mammalian SWI-SNF-like chromatin remodeling complexes and the vitamin D receptor (VDR) heterodimer. Mediates critical interactions between nuclear receptors and the BRG1/SMARCA4 chromatin-remodeling complex for transactivation. Also involved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR-mediated transrepression of the CYP27B1 gene.
  • 組織特異性
    Expressed in all tissues tested, including brain, heart, kidney, liver, lung, muscle, pancreas and placenta.
  • 配列類似性
    Belongs to the SMARCD family.
    Contains 1 SWIB domain.
  • 細胞内局在
    Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 60 kDa BRG-1/Brm-associated factor subunit A antibody
    • 60 kDa BRG1/Brm associated factor subunit A antibody
    • BAF60A antibody
    • BRG1 associated factor 60A antibody
    • BRG1-associated factor 60A antibody
    • Chromatin remodeling complex BAF60A subunit antibody
    • CRACD1 antibody
    • Mammalian chromatin remodeling complex BRG1 associated factor 60A antibody
    • Rsc6p antibody
    • SMARCD1 antibody
    • SMRD1 antibody
    • SMRD1_HUMAN antibody
    • SWI/SNF complex 60 kDa subunit A antibody
    • SWI/SNF complex 60 kDa subunit antibody
    • SWI/SNF related matrix associated actin dependent regulator of chromatin d1 antibody
    • SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily d, member 1 antibody
    • SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily D member 1 antibody
    • Swp73 like protein antibody
    see all

画像

  • All lanes : Anti-SMARCD1 antibody (ab86029) at 1/1000 dilution

    Lane 1 : An un-induced culture of E. coli
    Lane 2 : An induced culture of E. coli

    Lysates/proteins at 1/5000 dilution per lane.

    Secondary
    Anti-Rabbit IgG HRP at 1/1000 dilution

    Predicted band size : 58 kDa
    Observed band size : 72 kDa (why is the actual band size different from the predicted?)


    Exposure time : 60 minutes

プロトコール

参考文献

This product has been referenced in:
  • Chen D  et al. miR-100 induces epithelial-mesenchymal transition but suppresses tumorigenesis, migration and invasion. PLoS Genet 10:e1004177 (2014). WB ; Human . Read more (PubMed: 24586203) »

See 1 Publication for this product

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