The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml.
Use a concentration of 1 µg/ml. Detects a band of approximately 150 kDa (predicted molecular weight: 122 kDa).
1/2000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). May stimulate the ATPase activity of the catalytic subunit of the complex. Also involved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR-mediated transrepression of the CYP27B1 gene. Belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). During neural development a switch from a stem/progenitor to a post-mitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to post-mitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth.
Expressed in brain, heart, muscle, placenta, lung, liver, muscle, kidney and pancreas.
Belongs to the SMARCC family. Contains 1 SANT domain. Contains 1 SWIRM domain.
Phosphorylated on undefined residues at the G2/M transition by ERK1 and other kinases. This may contribute to cell cycle specific inactivation of remodeling complexes containing the phosphorylated protein.
SWI/SNF related matrix associated actin dependent regulator of chromatin c1 antibody
SWI/SNF related matrix associated actin dependent regulator of chromatin subfamily c member 1 antibody
SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily C member 1 antibody
Western blot - SMARCC1 antibody (ab22355)
All lanes : Anti-SMARCC1 antibody (ab22355) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 20 ug
Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 20 ug with Human SMARCC1 peptide (ab24351) at 1 µg/ml
Secondary All lanes : Goat polyclonal to Rabbit IgG (Alexa Fluor® 680) at 1/10000 dilution
Predicted band size: 122 kDa Observed band size: 150 kDa (why is the actual band size different from the predicted?) Additional bands at: 100 kDa (possible cleavage fragment), 100 kDa (possible cross reactivity), 40 kDa (possible cleavage fragment), 40 kDa (possible cross reactivity), 85 kDa (possible cleavage fragment), 85 kDa (possible cross reactivity)
ab22355 detects a band at 150 kDa which corresponds in size to that seen for SMARCC1, which was originally identified as a ~150 kDa polypeptide and termed BAF155. It also detects some smaller proteins at approximately 100, 85 and 40 kDa which may be degradation products. All of these bands are competed away by the addition of the immunizing peptide, suggesting that the interaction is specific.
ICC/IF image of ab22355 stained human HeLa cells. The cells were methanol fixed (5 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab22355, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
ab22355 staining SMARCC1in human kidney. Paraffin embedded human kidney tissue was incubated with ab22355 (1/2000 dilution) for 30 mins at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH 6.
ab22355 was tested in a tissue microarray (TMA) containing a wide range of normal and cancer tissues as well as a cell microarray consisting of a range of commonly used, well characterised human cell lines.