製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to SmarcAL1
  • 特異性
    This antibody detects a band at 105kD which corresponds to SmarcAL1. It also detects several lower weight bands of unknown identity.
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide:

    SNADQRPHDSHSFQAKGC

    , corresponding to amino acids 80-96 of Human SmarcAL1.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファー
    Preservative: 0.01% Sodium Azide
    Constituents: 0.15M Sodium Chloride, 0.02M Potassium Phosphate. pH 7.2
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab4743 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500. Detects a band of approximately 105 kDa (predicted molecular weight: 105 kDa).

ターゲット情報

  • 機能
    ATP-dependent annealing helicase that catalyzes the rewinding of the stably unwound DNA. Rewinds single-stranded DNA bubbles that are stably bound by replication protein A (RPA). Acts throughout the genome to reanneal stably unwound DNA, performing the opposite reaction of many enzymes, such as helicases and polymerases, that unwind DNA.
  • 組織特異性
    Ubiquitously expressed, with high levels in testis.
  • 関連疾患
    Defects in SMARCAL1 are a cause of Schimke immuno-osseous dysplasia (SIOD) [MIM:242900]. SIOD causes spondyloepiphyseal dysplasia, renal dysfunction and T-cell immunodeficiency. Approximately half of all patients also exhibit hyperthyroidism, while around half also exhibit episodal cerebral ischema.
  • 配列類似性
    Belongs to the SNF2/RAD54 helicase family. SMARCAL1 subfamily.
    Contains 2 HARP domains.
    Contains 1 helicase ATP-binding domain.
    Contains 1 helicase C-terminal domain.
  • 細胞内局在
    Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • HARP antibody
    • HepA Related Protein antibody
    • HepA-related protein antibody
    • hHARP antibody
    • SIOD antibody
    • SMAL1_HUMAN antibody
    • SMARCA like Protein 1 antibody
    • smarcal1 antibody
    • Sucrose nonfermenting protein 2 like 1 antibody
    • Sucrose nonfermenting protein 2-like 1 antibody
    • SWI/SNF Related antibody
    • SWI/SNF related matrix associated actin dependent regulator of chromatin subfamily A like protein 1 antibody
    • SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A-like protein 1 antibody
    see all

画像



  • Predicted band size : 105 kDa


    Western blot using ab4743 at 1/500.

    Lane 1: Hela nuclear
    Lane 2: Hela
    Lane 3: A431
    Lane 4: Jurkat
    Lane 5: 293

    SmarcAL1 is the band seen between the 97 and 116kD markers.

    Secondary antibody: Goat polyclonal to Rabbit IgG H&L (HRP) Pre-Adsorbed ab7090 (1/5000)
    Exposure time: 1 minute
    Cell lysates: 5µg per lane.

プロトコール

参考文献

ab4743 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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