製品の概要

  • 製品名Anti-SMARCAD1 antibody
    SMARCAD1 一次抗体 製品一覧
  • 製品の詳細
    Mouse polyclonal to SMARCAD1
  • アプリケーション適用あり: WB, ICC/IFmore details
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    Full length SMARCAD1 protein (Human)

  • ポジティブ・コントロール
    • SMARCAD1 transfected 293T cell lysate

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファーPreservative: None
    Constituents: 1X PBS, pH 7.2
  • Concentration information loading...
  • 精製度Protein A purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab67548 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/1000. Detects a band of approximately 90 kDa (predicted molecular weight: 117 kDa).
ICC/IF 1/250.

ターゲット情報

  • 機能Probable ATP-dependent DNA helicase.
  • 組織特異性Ubiquitous.
  • 配列類似性Belongs to the SNF2/RAD54 helicase family.
    Contains 2 CUE domains.
    Contains 1 helicase ATP-binding domain.
    Contains 1 helicase C-terminal domain.
  • 翻訳後修飾Phosphorylated upon DNA damage, probably by ATM or ATR.
  • 細胞内局在Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • ATP dependent helicase 1 antibody
    • ATP-dependent helicase 1 antibody
    • ETL1 antibody
    • hHEL1 antibody
    • Smarcad1 antibody
    • SMRCD_HUMAN antibody
    • SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A containing DEAD/H box 1 antibody
    see all

Anti-SMARCAD1 antibody 画像

  • All lanes : Anti-SMARCAD1 antibody (ab67548) at 1/500 dilution

    Lane 1 : SMARCAD1 transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 117 kDa
    Observed band size : 90 kDa (why is the actual band size different from the predicted?)
  • 4-cell stage mouse embryos were recovered and the zona pellucida removed. ab67548 at 1:250 dilution was used to detect SMARCAD1, and as secondary antibldy embryos were incubated in Alexa-fluor488 conjugated donkey anti-mouse IgG. Embryos were then imaged by fluorescence confocal microscopy and projected confocal stack images generated - as shown in two examples. Please see accompanying abreview for additional information.

    See Abreview

Anti-SMARCAD1 antibody (ab67548) 使用論文

ab67548 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (Pre-implantation mouse embryos (4 cell stage))
Specification Pre-implantation mouse embryos (4 cell stage)
Fixative Paraformaldehyde
Permeabilization Yes - 0.5 TritonX-100
Blocking step BSA as blocking agent for 4 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 4°C
Username

Dr. Alex Bruce

Verified customer

投稿 Apr 09 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"