製品の概要

  • 製品名Anti-SMARCA2 / BRM antibody
    SMARCA2 / BRM 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to SMARCA2 / BRM
  • アプリケーション適用あり: WB, ICC/IFmore details
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Rat, Chicken, Xenopus laevis, Zebrafish
  • 免疫原

    Synthetic peptide:

    KEDVEKAKKRRGRPPAE

    , corresponding to amino acids 1354-1370 of SMARCA2 conjugated to maleimide-activated KLH via an N-terminal added cysteine.

  • ポジティブ・コントロール
    • WB: Nuclear extracts of SMARCA2 transfected 293T cell line. ICC: Paraformaldehyde/Triton fixed SMARCA2 transfected 293T cell line.
  • 特記事項Storage in frost-free freezers is also not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab12165 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB
ICC/IF
  • 追加情報IF(ICC): Use at a concentration of 1 µg/ml on paraformaldehyde/Triton fixed SMARCA2-transfected 293T cell line). Indirect IF.
    WB: Use at a concentration of 0.15 µg/ml (determined using nuclear
    extracts of hBrm-transfected 293T cells). Predicted molecular weight: 230 kDa. Staining of SMARCA2 is specifically inhibited by the immunizing peptide.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能Transcriptional coactivator cooperating with nuclear hormone receptors to potentiate transcriptional activation. Also involved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR-mediated transrepression of the CYP27B1 gene. Belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). During neural development a switch from a stem/progenitor to a post-mitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to post-mitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth.
    • 関連疾患Nicolaides-Baraitser syndrome
    • 配列類似性Belongs to the SNF2/RAD54 helicase family.
      Contains 1 bromo domain.
      Contains 1 helicase ATP-binding domain.
      Contains 1 helicase C-terminal domain.
      Contains 1 HSA domain.
      Contains 1 QLQ domain.
    • 細胞内局在Nucleus.
    • Information by UniProt
    • 参照データベース
    • 別名
      • ATP dependent helicase SMARCA2 antibody
      • ATP-dependent helicase SMARCA2 antibody
      • BAF190 antibody
      • BAF190B antibody
      • BRG1-associated factor 190B antibody
      • BRM antibody
      • FLJ36757 antibody
      • Global transcription activator homologous sequence antibody
      • hBRM antibody
      • hSNF2a antibody
      • MGC74511 antibody
      • Possible global transcription activator SNF2L2 antibody
      • Probable global transcription activator SNF2L2 antibody
      • Protein brahma homolog antibody
      • SMARCA2 antibody
      • SMCA2_HUMAN antibody
      • SNF2 alpha antibody
      • SNF2 like 2 antibody
      • SNF2-alpha antibody
      • SNF2/SWI2 like protein 2 antibody
      • SNF2L2 antibody
      • SNF2LA antibody
      • Sth1p antibody
      • Sucrose nonfermenting 2 like protein 2 antibody
      • SWI/SNF related matrix associated actin dependent regulator of chromatin subfamily A member 2 antibody
      • SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 2 antibody
      see all

    Anti-SMARCA2 / BRM antibody (ab12165) 使用論文

    This product has been referenced in:
    • Nan X  et al. Interaction between chromatin proteins MECP2 and ATRX is disrupted by mutations that cause inherited mental retardation. Proc Natl Acad Sci U S A 104:2709-14 (2007). WB ; Mouse . Read more (PubMed: 17296936) »

    See 1 Publication for this product

    Product Wall

    There are currently no Abreviews or Questions for ab12165.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"