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A region within synthetic peptide: GAAAAATEGT TATEKGEKKE KITSPFQLKL AAKASKSEKE MDPEYEEKMV, corresponding to N terminal amino acids 38-87 of Mouse SMARCA1
Our Abpromise guarantee covers the use of ab49126 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ChIP||Use at an assay dependent concentration.|
|ELISA||Use at an assay dependent concentration.
ELISA titre using peptide based assay was 1:312500.
|WB||Use a concentration of 1.25 µg/ml. Detects a band of approximately 43 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.|
ChIP analysis using ab49126 (0.5µg/mL) binding SMARCA1 in human HCT116 colon cancer cells. Without (- peptide) and with (+ peptide) blocking peptide. Protein binding was detected using real-time PCR.
ab49126 has not yet been referenced specifically in any publications.