製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to SMAP2
  • アプリケーション
    適用あり: IHC-P, ICC/IF, WBmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    antigen sequence, corresponding to C terminal amino acids 326-429 of Human SMAP2.

  • ポジティブ・コントロール
    • Human lymph node tissue; A431 cells; RT 4, U 251 MG, Human Liver and Tonsil cell lysates.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab121675 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P 1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use a concentration of 1 - 4 µg/ml. Fixation/Permeabilization: PFA/Triton X-100
WB 1/250 - 1/500.

ターゲット情報

  • 機能
    GTPase activating protein that acts on ARF1. Can also activate ARF6 (in vitro). May play a role in clathrin-dependent retrograde transport from early endosomes to the trans-Golgi network.
  • 配列類似性
    Contains 1 Arf-GAP domain.
  • 細胞内局在
    Cytoplasm. Detected in multiple foci throughout the cytoplasm and in juxtanuclear structures.
  • Information by UniProt
  • 参照データベース
  • 別名
    • RP1-228H13.3 antibody
    • Small ArfGAP2 antibody
    • SMAP 1L antibody
    • Smap2 antibody
    • SMAP2_HUMAN antibody
    • Stromal membrane associated GTPase activating protein 2 antibody
    • Stromal membrane-associated protein 1-like antibody
    • Stromal membrane-associated protein 2 antibody
    see all

画像

  • Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells)
    Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells)
  • All lanes : Anti-SMAP2 antibody (ab121675) at 1/250 dilution

    Lane 1 : RT 4 lysate
    Lane 2 : U 251 MG lysate
    Lane 3 : Human plasma lysate
    Lane 4 : Human Liver lysate
    Lane 5 : Human tonsil lysate

    Developed using the ECL technique
  • ab121675 at 1/300 dilution staining SMAP2 in Paraffin-embedded Human lymph node tissue by Immunohistochemistry.
  • ab121675 at 4 µg/ml staining SMAP2 in PFA/Triton X-100 fixed/permeabilized A431 cells by Immunofluorescence (green).

参考文献

ab121675 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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