製品の概要

  • 製品名Anti-SMAD6 antibody
    SMAD6 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to SMAD6
  • アプリケーション適用あり: WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Chimpanzee
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Human SMAD6.

    (Peptide available as ab66351.)

  • ポジティブ・コントロール
    • This antibody gave a positive signal in the following Human Whole Cell Lysates: HeLa, Jurkat, A431, HEK293, HepG2, MCF7, SHSY-5Y and U2OS.

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab63713 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 52 kDa (predicted molecular weight: 52 kDa).

ターゲット情報

  • 機能Antagonist of signaling by TGF-beta (transforming growth factor) type 1 receptor superfamily members; has been shown to inhibit selectively BMP (bone morphogenetic proteins) signaling by competing with the co-SMAD SMAD4 for receptor-activated SMAD1. SMAD6 is an inhibitory SMAD (I-SMAD) or antagonistic SMAD. Binds to regulatory elements in target promoter regions.
  • 組織特異性Ubiquitous in various organs, with higher levels in lung. Isoform B is up-regulated in diseased heart tissue.
  • 配列類似性Belongs to the dwarfin/SMAD family.
    Contains 1 MH1 (MAD homology 1) domain.
    Contains 1 MH2 (MAD homology 2) domain.
  • 翻訳後修飾Phosphorylated by BMP type 1 receptor kinase and by PRKX.
    Ubiquitinated by WWP1.
  • 細胞内局在Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • hSMAD6 antibody
    • MAD homolog 6 antibody
    • MADH6 antibody
    • MADH7 antibody
    • Mothers against decapentaplegic homolog 6 antibody
    • Mothers against DPP homolog 6 antibody
    • SMAD 6 antibody
    • SMAD family member 6 antibody
    • SMAD mothers against DPP homolog 6 antibody
    • Smad6 antibody
    • SMAD6_HUMAN antibody
    see all

Anti-SMAD6 antibody 画像

  • All lanes : Anti-SMAD6 antibody (ab63713) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
    Lane 3 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 4 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
    Lane 5 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 6 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
    Lane 7 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate
    Lane 8 : U2OS (Human osteosarcoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size : 52 kDa
    Observed band size : 52 kDa
    Additional bands at : 130 kDa,70 kDa. We are unsure as to the identity of these extra bands.

Anti-SMAD6 antibody (ab63713) 使用論文

This product has been referenced in:
  • Chen RY  et al. Effect of oridonin-mediated hallmark changes on inflammatory pathways in human pancreatic cancer (BxPC-3) cells. World J Gastroenterol 20:14895-903 (2014). WB ; Human . Read more (PubMed: 25356049) »

See 1 Publication for this product

Product Wall

Application Western blot
Sample Human Cell lysate - whole cell (Isolated Primary NK Cells)
Gel Running Conditions Reduced Denaturing
Loading amount 250000 cells
Treatment Rest, IL15, TGFB, IL12, IL18 for 24 hrs
Specification Isolated Primary NK Cells
Blocking step Milk as blocking agent for 24 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Sian Drake

Verified customer

投稿 May 23 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"