Anti-SMAD5 (phospho S463 + S465) 抗体 [EP728(2)AY] (ab76296)

製品の概要

  • 製品名Anti-SMAD5 (phospho S463 + S465) antibody [EP728(2)AY]
    SMAD5 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EP728(2)AY] to SMAD5 (phospho S463 + S465)
  • 特異性Detects SMAD5 phosphorylated at Serine 463 and 465. This antibody may cross-react with Smad1 Phospho (pS463/465) and Smad9 Phospho (pS465/467).
  • アプリケーション適用あり: WB, ICC/IFmore details
    適用なし: Flow Cyt,IHC-P or IP
  • 種交差性
    交差種: Human
  • 免疫原

    A phospho specific peptide corresponding to residues surrounding Serine 463 and 465 of human SMAD5.

  • ポジティブ・コントロール
    • HeLa cytoplasmic or nuclear lysate treated with BMP-4 HeLa cells
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

     

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab76296 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500. Predicted molecular weight: 52 kDa.
ICC/IF 1/50 - 1/100.
  • 追加情報Is unsuitable for Flow Cyt,IHC-P or IP.
  • ターゲット情報

    • 機能Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD5 is a receptor-regulated SMAD (R-SMAD).
    • 組織特異性Ubiquitous.
    • 配列類似性Belongs to the dwarfin/SMAD family.
      Contains 1 MH1 (MAD homology 1) domain.
      Contains 1 MH2 (MAD homology 2) domain.
    • 翻訳後修飾Phosphorylated on serine by BMP (bone morphogenetic proteins) type 1 receptor kinase.
      Ubiquitin-mediated proteolysis by SMAD-specific E3 ubiquitin ligase SMURF1.
    • 細胞内局在Cytoplasm. Nucleus. Cytoplasmic in the absence of ligand. Migrates to the nucleus when complexed with SMAD4.
    • Information by UniProt
    • 参照データベース
    • 別名
      • DKFZp781C1895 antibody
      • DKFZp781O1323 antibody
      • Dwfc antibody
      • hSmad5 antibody
      • JV5 1 antibody
      • JV5-1 antibody
      • MAD homolog 5 antibody
      • MAD, mothers against decapentaplegic homolog 5 antibody
      • MADH 5 antibody
      • MADH5 antibody
      • Mothers against decapentaplegic homolog 5 antibody
      • mothers against decapentaplegic, drosophila, homolog of, 5 antibody
      • Mothers against DPP homolog 5 antibody
      • MusMLP antibody
      • SMA and MAD related protein 5 antibody
      • SMAD 5 antibody
      • SMAD family member 5 antibody
      • SMAD, mothers against DPP homolog 5 antibody
      • Smad5 antibody
      • SMAD5_HUMAN antibody
      see all

    Anti-SMAD5 (phospho S463 + S465) antibody [EP728(2)AY] 画像

    • All lanes : Anti-SMAD5 (phospho S463 + S465) antibody [EP728(2)AY] (ab76296) at 1/500 dilution

      Lane 1 : HeLa cell lysate - untreated
      Lane 2 : HeLa cell lysate - treated with BMP-4

      Lysates/proteins at 10 µg per lane.

      Secondary
      goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size : 52 kDa
      Observed band size : 52 kDa
    • Immunofluorescent staining of HeLa cells using ab76296 at a 1/50-1/100 dilution.

    Anti-SMAD5 (phospho S463 + S465) antibody [EP728(2)AY] (ab76296) 使用論文

    This product has been referenced in:
    • Ghiabi P  et al. Breast cancer cells promote a notch-dependent mesenchymal phenotype in endothelial cells participating to a pro-tumoral niche. J Transl Med 13:27 (2015). WB ; Human . Read more (PubMed: 25623554) »
    • Nili M  et al. Soluble Repulsive Guidance Molecule c/Hemojuvelin Is a Broad Spectrum Bone Morphogenetic Protein (BMP) Antagonist and Inhibits both BMP2- and BMP6-mediated Signaling and Gene Expression. J Biol Chem 285:24783-92 (2010). WB ; Human . Read more (PubMed: 20530805) »

    See all 2 Publications for this product

    Product Wall

    Application Western blot
    Sample Human Cell lysate - whole cell (Human prostate and cervical cancer cell)
    Gel Running Conditions Reduced Denaturing (7.5%)
    Loading amount 30 µg
    Specification Human prostate and cervical cancer cell
    Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    投稿 Aug 20 2015

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"