Anti-SMAD5 抗体 (ab88559)
Key features and details
- Rabbit polyclonal to SMAD5
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-SMAD5 antibody
SMAD5 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to SMAD5 -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat, Orangutan -
免疫原
Synthetic peptide corresponding to Human SMAD5 aa 100-200 conjugated to keyhole limpet haemocyanin.
(Peptide available asab100821) -
ポジティブ・コントロール
- This antibody gave a positive signal in the following whole cell lysates: HeLa; HepG2; MCF7.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab88559の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 52 kDa (predicted molecular weight: 52 kDa).
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特記事項 |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 52 kDa (predicted molecular weight: 52 kDa). |
ターゲット情報
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機能
Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD5 is a receptor-regulated SMAD (R-SMAD). -
組織特異性
Ubiquitous. -
配列類似性
Belongs to the dwarfin/SMAD family.
Contains 1 MH1 (MAD homology 1) domain.
Contains 1 MH2 (MAD homology 2) domain. -
翻訳後修飾
Phosphorylated on serine by BMP (bone morphogenetic proteins) type 1 receptor kinase.
Ubiquitin-mediated proteolysis by SMAD-specific E3 ubiquitin ligase SMURF1. -
細胞内局在
Cytoplasm. Nucleus. Cytoplasmic in the absence of ligand. Migrates to the nucleus when complexed with SMAD4. - Information by UniProt
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参照データベース
- Entrez Gene: 4090 Human
- Entrez Gene: 17129 Mouse
- Entrez Gene: 59328 Rat
- Omim: 603110 Human
- SwissProt: Q99717 Human
- SwissProt: P97454 Mouse
- SwissProt: Q9R1V3 Rat
- Unigene: 167700 Human
see all -
別名
- DKFZp781C1895 antibody
- DKFZp781O1323 antibody
- Dwfc antibody
see all
画像
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All lanes : Anti-SMAD5 antibody (ab88559) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 3 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 52 kDa
Observed band size: 52 kDa
Exposure time: 4 minutes
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (3)
ab88559 は 3 報の論文で使用されています。
- Yuan W et al. Long non-coding RNA LINC01116 accelerates the progression of keloid formation by regulating miR-203/SMAD5 axis. Burns 47:665-675 (2021). PubMed: 32883538
- Xue D et al. Circ-AKT3 inhibits clear cell renal cell carcinoma metastasis via altering miR-296-3p/E-cadherin signals. Mol Cancer 18:151 (2019). PubMed: 31672157
- Dai J et al. Genistein promotion of osteogenic differentiation through BMP2/SMAD5/RUNX2 signaling. Int J Biol Sci 9:1089-98 (2013). WB . PubMed: 24339730