製品の概要

  • 製品名
    Anti-Smad2 + Smad3 antibody
    Smad2 + Smad3 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Smad2 + Smad3
  • 特異性
    ab63672 detects endogenous levels of total Smad2 and Smad3.
  • アプリケーション
    適用あり: ELISA, WBmore details
  • 種交差性
    交差種: Rat, Human
    交差が予測される動物種: Mouse
  • 免疫原

    Synthetic non-phosphopeptide derived from human Smad2 and Smad3 around the phosphorylation site of threonine 8 (P-F-TP-P-P).

  • ポジティブ・コントロール
    • COS7 cells.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab63672 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA 1/5000.
WB 1/500 - 1/1000. Detects a band of approximately 48 kDa.

ターゲット情報

  • 関連性
    SMAD is a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. They mediate the signal of the transforming growth factor (TGF)-beta, and thus regulate multiple cellular processes, such as cell proliferation, apoptosis, and differentiation.
  • 細胞内局在
    Cytoplasm. Nucleus. Note: Cytoplasmic in the absence of ligand. Migrates to the nucleus when complexed with SMAD4.
  • 参照データベース
  • 別名
    • hMAD 2 antibody
    • hMAD 3 antibody
    • hSMAD2 antibody
    • hSMAD3 antibody
    • Mad related protein 2 antibody
    • MADH2 antibody
    • MADH3 antibody
    • MADR2 antibody
    • Mothers against DPP homolog 2 antibody
    • Mothers against DPP homolog 3 antibody
    • Sma and Mad related protein 2 antibody
    • SMA and MAD related protein 3 antibody
    • SMAD 2 antibody
    • SMAD 3 antibody
    • SMAD family member 2 antibody
    • SMAD family member 3 antibody
    • smad2+3 antibody
    • smad2/3 antibody
    see all

Anti-Smad2 + Smad3 antibody 画像

  • All lanes : Anti-Smad2 + Smad3 antibody (ab63672) at 1/500 dilution

    Lane 1 : COS7 cell extracts treated with UV (15mins)
    Lane 2 : COS7 cell extracts untreated
    Lane 3 : Jurkat cell extracts treated with EGF (200ng/ml, 30mins)
    Lane 4 : Jurkat cell extracts treated with EGF (200ng/ml, 30mins) with immunizing peptide at 10 µg

    Lysates/proteins at 30 µg per lane.


    Observed band size : 48 kDa (why is the actual band size different from the predicted?)

プロトコール

Anti-Smad2 + Smad3 antibody (ab63672) 使用論文

This product has been referenced in:
  • Chen H  et al. Shenqiwan Ameliorates Renal Fibrosis in Rats by Inhibiting TGF-ß1/Smads Signaling Pathway. Evid Based Complement Alternat Med 2017:7187038 (2017). WB ; Rat . Read more (PubMed: 28638433) »
  • Wei MG  et al. JiaWeiDangGui Decoction Ameliorates Proteinuria and Kidney Injury in Adriamycin-Induced Rat by Blockade of TGF-ß/Smad Signaling. Evid Based Complement Alternat Med 2016:5031890 (2016). WB ; Rat . Read more (PubMed: 27403197) »

See all 4 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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