The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/1000. Detects a band of approximately 48 kDa.
SMAD is a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. They mediate the signal of the transforming growth factor (TGF)-beta, and thus regulate multiple cellular processes, such as cell proliferation, apoptosis, and differentiation.
Note: Cytoplasmic in the absence of ligand. Migrates to the nucleus when complexed with SMAD4.
Western blot - Anti-Smad2 + Smad3 antibody (ab63672)
All lanes : Anti-Smad2 + Smad3 antibody (ab63672) at 1/500 dilution
Lane 1 : COS7 cell extracts treated with UV (15mins) Lane 2 : COS7 cell extracts untreated Lane 3 : Jurkat cell extracts treated with EGF (200ng/ml, 30mins) Lane 4 : Jurkat cell extracts treated with EGF (200ng/ml, 30mins) with immunizing peptide at 10 µg
Wei MG et al. JiaWeiDangGui Decoction Ameliorates Proteinuria and Kidney Injury in Adriamycin-Induced Rat by Blockade of TGF-ß/Smad Signaling. Evid Based Complement Alternat Med2016:5031890 (2016).
Read more (PubMed: 27403197) »
Wei MG et al. Ferulic Acid Attenuates TGF-ß1-Induced Renal Cellular Fibrosis in NRK-52E Cells by Inhibiting Smad/ILK/Snail Pathway. Evid Based Complement Alternat Med2015:619720 (2015).
Read more (PubMed: 25949265) »