Anti-Smad2 + Smad3 抗体 (ab63672)
Key features and details
- Rabbit polyclonal to Smad2 + Smad3
- Suitable for: WB
- Reacts with: Human, African green monkey
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-Smad2 + Smad3 antibody
Smad2 + Smad3 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Smad2 + Smad3 -
由来種
Rabbit -
特異性
ab63672 detects endogenous levels of total Smad2 and Smad3. -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human, African green monkey
交差が予測される動物種: Mouse -
免疫原
Synthetic non-phosphopeptide derived from human Smad2 and Smad3 around the phosphorylation site of threonine 8 (P-F-TP-P-P).
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol, 0.87% Sodium chloride
Without Mg2+ and Ca2+ -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab63672の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/500 - 1/1000. Detects a band of approximately 48 kDa.
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特記事項 |
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WB
1/500 - 1/1000. Detects a band of approximately 48 kDa. |
ターゲット情報
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関連性
SMAD is a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. They mediate the signal of the transforming growth factor (TGF)-beta, and thus regulate multiple cellular processes, such as cell proliferation, apoptosis, and differentiation. -
細胞内局在
Cytoplasm. Nucleus. Note: Cytoplasmic in the absence of ligand. Migrates to the nucleus when complexed with SMAD4. -
参照データベース
- Entrez Gene: 4087 Human
- Entrez Gene: 4088 Human
- Entrez Gene: 17126 Mouse
- Entrez Gene: 17127 Mouse
- Omim: 601366 Human
- Omim: 603109 Human
- SwissProt: P84022 Human
- SwissProt: Q15796 Human
see all -
別名
- hMAD 2 antibody
- hMAD 3 antibody
- hSMAD2 antibody
see all
画像
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All lanes : Anti-Smad2 + Smad3 antibody (ab63672) at 1/500 dilution
Lane 1 : COS-7 (African green monkey kidney fibroblast-like cell line) cell extracts treated with UV (15mins)
Lane 2 : COS-7 (African green monkey kidney fibroblast-like cell line) cell extracts untreated
Lane 3 : Jurkat cell extracts treated with EGF (200ng/ml, 30mins)
Lane 4 : Jurkat cell extracts treated with EGF (200ng/ml, 30mins) with immunizing peptide at 10 µg
Lysates/proteins at 30 µg per lane.
Observed band size: 48 kDa why is the actual band size different from the predicted?
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (16)
ab63672 は 16 報の論文で使用されています。
- Yamamoto A et al. Therapeutic Effect of Cyclin-Dependent Kinase 4/6 Inhibitor on Dermal Fibrosis in Murine Models of Systemic Sclerosis. Arthritis Rheumatol 74:860-870 (2022). PubMed: 34882985
- Liao CG et al. Active demethylation upregulates CD147 expression promoting non-small cell lung cancer invasion and metastasis. Oncogene 41:1780-1794 (2022). PubMed: 35132181
- Yin J et al. Isoflurane Postconditioning Upregulates Phosphorylated Connexin 43 in the Middle Cerebral Artery Occlusion Model and Is Probably Associated with the TGF-ß1/Smad2/3 Signaling Pathway. Biomed Res Int 2020:3451215 (2020). PubMed: 32258113
- Zong M et al. Irbesartan ameliorates myocardial fibrosis in diabetic cardiomyopathy rats by inhibiting the TGFß1/Smad2/3 pathway. Exp Ther Med 20:117 (2020). PubMed: 33005243
- Wang T et al. A novel tsRNA-16902 regulating the adipogenic differentiation of human bone marrow mesenchymal stem cells. Stem Cell Res Ther 11:365 (2020). PubMed: 32831139