Anti-SMAD1/3/5 (phospho S423 + S425) 抗体 (ab51451)

製品の概要

  • 製品名
    Anti-SMAD1/3/5 (phospho S423 + S425) antibody
  • 製品の詳細
    Rabbit polyclonal to SMAD1/3/5 (phospho S423 + S425)
  • 特異性
    ab51451 is specific for the phosphorylated form of the protein although minimal reactivity with nonphosphorylated human Smad3 can be observed by ELISA and WB. ab51451 also reacts with phosphorylated Smad1 and Smad5 but negligible reactivity is seen against other phosphorylated Smad family members.
  • アプリケーション
    適用あり: IHC-P, WB, ELISA, ICC/IFmore details
  • 種交差性
    交差種: Mouse, Chicken, Human
    交差が予測される動物種: Rat, Cow, Pig, Xenopus laevis, Zebrafish
  • 免疫原

    Synthetic peptide corresponding to Human SMAD1/3/5 aa 417-425.
    Sequence:

    PSIRCSSVS

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab51451 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use at an assay dependent concentration. PubMed: 20519446
WB 1/2000 - 1/20000. Predicted molecular weight: 48 kDa. Stimulation with 2 ng/ml TGF-beta for 1 hour is suggested.
ELISA 1/25000 - 1/100000.
ICC/IF Use at an assay dependent concentration.

ターゲット情報

Anti-SMAD1/3/5 (phospho S423 + S425) antibody 画像

  • All lanes : Anti-SMAD1/3/5 (phospho S423 + S425) antibody (ab51451) at 1 µg/ml

    Lane 1 : HEK293 lysate
    Lane 2 : HeLa Lysate
    Lane 3 : MCF-7 Lysate
    Lane 4 : Jurkat Lysate
    Lane 5 : A431 Lysate
    Lane 6 : A549 Lysate
    Lane 7 : LNCap Lysate
    Lane 8 : MOLT-4 Lysate
    Lane 9 : Ramos Lysate
    Lane 10 : Raji Lysate
    Lane 11 : A-172 Lysate
    Lane 12 : NIH/3T3 Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Lanes 1, 2, 4, 5, 6, 7, 8, 9, 10, 11 & 12 : Peroxidase rabbit secondary antibody at 1/30000 dilution
    Lane 3 : Peroxidase rabbit secondary antibody at 30000


    Predicted band size : 48 kDa
  • ab51451 at 2.5 µg/ml staining epidermal keratinocytes at 40X.  This image shows strong nuclear staining with localization of the antibody as the precipated red siganl and a hematoxylin purple nuclear counterstain.

  • ab51451 staining Smad3 (phospho S423 + S425) in PC3 and LnCAP cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 70% ethanol and blocked for 1 hour at 26°C. Samples were incubated with primary antibody (1/150) for 16 hours at 4°C. An IRDye® 800CW-conjugated goat anti-rabbit IgG polyclonal (1/500) was used as the secondary antibody. NC-PC3 - negative control PC3 cells, NC-LnCAP - negative control LnCAP cells.

    See Abreview

  • All lanes : Anti-SMAD1/3/5 (phospho S423 + S425) antibody (ab51451) at 1/2000 dilution

    Lane 1 : MEF lysate (control)
    Lane 2 : MEF lysate, after stimulation with 2 ng/ml of TGF-ß for 1 hour
    Lane 3 : MEF-/-Smad3 lysate (control)
    Lane 4 : MEF-/-Smad3 lysate, after stimulation with 2 ng/ml of TGF-ß for one hour
    Lane 5 : Hep3B lysate (control)
    Lane 6 : Hep3B lysate, after stimulation with 2 ng/ml of TGF-ß for one hour
    Lane 7 : HEK 293 lysate (control)


    Predicted band size : 48 kDa
    Image courtesy of Ying Zhang, NIH, CCR, Bethesda, MD.

Anti-SMAD1/3/5 (phospho S423 + S425) antibody (ab51451) 使用論文

This product has been referenced in:
  • Davaapil H  et al. Conserved and novel functions of programmed cellular senescence during vertebrate development. Development 144:106-114 (2017). WB . Read more (PubMed: 27888193) »
  • Gardenier JC  et al. Topical tacrolimus for the treatment of secondary lymphedema. Nat Commun 8:14345 (2017). IHC-P ; Mouse . Read more (PubMed: 28186091) »

See all 21 Publications for this product

Product Wall

Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (heart)
Permeabilization
Yes - 0.3% Triton X
Specification
heart
Blocking step
Serum as blocking agent for 20 minute(s) · Concentration: 20% · Temperature: 25°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

投稿 Oct 19 2015

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
Odyssey Blocking Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 26°C
Sample
Human Cell (PC3 and LnCAP)
Specification
PC3 and LnCAP
Permeabilization
Yes - 70% Ethanol
Fixative
Paraformaldehyde
Username

Dr. Francesco Elia Marino

Verified customer

投稿 May 02 2014

Application
Western blot
Loading amount
60 µg
Gel Running Conditions
Reduced Non-Denaturing (Native) (12)
Sample
Mouse Tissue lysate - whole (Mouse gastrocnemius)
Specification
Mouse gastrocnemius
Blocking step
Odyssey Blocking Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 26°C
Username

Dr. Francesco Elia Marino

Verified customer

投稿 Mar 26 2014

Thank you for calling Abcam earlier today.

As we talked about on the telephone, I am sending you ab52903 as a free of charge replacement. Your new order/reference number is********.

Please let me know if there is anything else I can...

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Thank you for your reply.

The only other phospho-smad3 (S423+S425) we have are:

ab11825 - http://www.abcam.com/Smad3-phospho-S423-S425-antibody-ab118825.html

ab52903 - http://www.abcam.com/Smad3-phospho-S423-S425-antibody...

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Thank you very much for contacting Abcam.

The isotype control that I would recommend for your flow cytometry experiments is:

http://www.abcam.com/Rabbit-IgG-ChIP-Grade-ab37415.html



Below is the infromation about...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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