Anti-Smad1 (phospho S465) 抗体 (ab97689)

製品の概要

  • 製品名Anti-Smad1 (phospho S465) antibody
    Smad1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Smad1 (phospho S465)
  • 特異性ab97689 detects endogenous levels of Smad1 only when phosphorylated at serine 465.
  • アプリケーション適用あり: WBmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic phosphopeptide derived from Human Smad1 around the phosphorylation site of serine 465 (I-S-S-V-SP).

  • ポジティブ・コントロール
    • HeLa cell extracts treated with PMA.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab97689 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/1000. Detects a band of approximately 55 kDa (predicted molecular weight: 52 kDa).

ターゲット情報

  • 機能Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD1 is a receptor-regulated SMAD (R-SMAD). SMAD1/OAZ1/PSMB4 complex mediates the degradation of the CREBBP/EP300 repressor SNIP1.
  • 組織特異性Ubiquitous. Highest expression seen in the heart and skeletal muscle.
  • 配列類似性Belongs to the dwarfin/SMAD family.
    Contains 1 MH1 (MAD homology 1) domain.
    Contains 1 MH2 (MAD homology 2) domain.
  • 翻訳後修飾Phosphorylated on serine by BMP type 1 receptor kinase.
    Ubiquitin-mediated proteolysis by SMAD-specific E3 ubiquitin ligase SMURF1.
  • 細胞内局在Cytoplasm. Nucleus. Cytoplasmic in the absence of ligand. Migrates to the nucleus when complexed with SMAD4. Co-localizes with LEMD3 at the nucleus inner membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • BSP-1 antibody
    • BSP1 antibody
    • HsMAD1 antibody
    • JV4-1 antibody
    • JV41 antibody
    • MAD homolog 1 antibody
    • MAD mothers against decapentaplegic homolog 1 antibody
    • Mad related protein 1 antibody
    • Mad-related protein 1 antibody
    • MADH1 antibody
    • MADR1 antibody
    • Mothers against decapentaplegic homolog 1 antibody
    • Mothers against DPP homolog 1 antibody
    • SMA- AND MAD-RELATED PROTEIN 1 antibody
    • SMAD 1 antibody
    • SMAD family member 1 antibody
    • SMAD mothers against DPP homolog 1 antibody
    • Smad1 antibody
    • SMAD1_HUMAN antibody
    • TGF beta signaling protein 1 antibody
    • Transforming growth factor-beta-signaling protein 1 antibody
    see all

Anti-Smad1 (phospho S465) antibody 画像

  • All lanes : Anti-Smad1 (phospho S465) antibody (ab97689) at 1/500 dilution

    Lane 1 : HeLa cell extracts, untreated
    Lane 2 : HeLa cell extracts, treated with PMA


    Predicted band size : 52 kDa

Anti-Smad1 (phospho S465) antibody (ab97689) 使用論文

ab97689 has not yet been referenced specifically in any publications.

Product Wall

Application Immunohistochemistry (Frozen sections)
Sample Xenopus laevis Tissue sections (st.10.5 embryo)
Permeabilization No
Specification st.10.5 embryo
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 22°C
Fixative Formaldehyde
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投稿 Jul 20 2016

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We cannot disclose our exact immunogen sequence of ab63439. However, the amino acid range of this immunogen is aa415-465 of human Mothers against decapentaplegic homolog 1 with ACC#Q15797 (http://www.uniprot.org/...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"