Anti-Smad1 (phospho S465) 抗体 (ab97689)
Key features and details
- Rabbit polyclonal to Smad1 (phospho S465)
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-Smad1 (phospho S465) antibody
Smad1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Smad1 (phospho S465) -
由来種
Rabbit -
特異性
ab97689 detects endogenous levels of Smad1 only when phosphorylated at serine 465. The region surrounding Smad1 phospho S465 is highly conserved in several other Smad proteins, including Smad 5 and Smad 9. Cross reactivity with other Smad proteins is likely. -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide corresponding to Human Smad1 (phospho S465).
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ポジティブ・コントロール
- HeLa cell extracts treated with PMA.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 0.87% Sodium chloride, 50% Glycerol (glycerin, glycerine), PBS
Without Mg2+ and Ca2+ -
Concentration information loading...
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精製度
Immunogen affinity purified -
特記事項(精製)
ab97689 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab97689の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/500 - 1/1000. Detects a band of approximately 55 kDa (predicted molecular weight: 52 kDa).
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特記事項 |
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WB
1/500 - 1/1000. Detects a band of approximately 55 kDa (predicted molecular weight: 52 kDa). |
ターゲット情報
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機能
Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD1 is a receptor-regulated SMAD (R-SMAD). SMAD1/OAZ1/PSMB4 complex mediates the degradation of the CREBBP/EP300 repressor SNIP1. -
組織特異性
Ubiquitous. Highest expression seen in the heart and skeletal muscle. -
配列類似性
Belongs to the dwarfin/SMAD family.
Contains 1 MH1 (MAD homology 1) domain.
Contains 1 MH2 (MAD homology 2) domain. -
翻訳後修飾
Phosphorylated on serine by BMP type 1 receptor kinase.
Ubiquitin-mediated proteolysis by SMAD-specific E3 ubiquitin ligase SMURF1. -
細胞内局在
Cytoplasm. Nucleus. Cytoplasmic in the absence of ligand. Migrates to the nucleus when complexed with SMAD4. Co-localizes with LEMD3 at the nucleus inner membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 4086 Human
- Omim: 601595 Human
- SwissProt: Q15797 Human
- Unigene: 604588 Human
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別名
- BSP-1 antibody
- BSP1 antibody
- HsMAD1 antibody
see all
画像
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (3)
ab97689 は 3 報の論文で使用されています。
- Onuma TA et al. A chordate species lacking Nodal utilizes calcium oscillation and Bmp for left-right patterning. Proc Natl Acad Sci U S A 117:4188-4198 (2020). PubMed: 32029598
- Zhang F et al. Anti-osteoporosis activity of Sanguinarine in preosteoblast MC3T3-E1 cells and an ovariectomized rat model. J Cell Physiol 233:4626-4633 (2018). PubMed: 28926099
- Waki K et al. Genetic pathways for differentiation of the peripheral nervous system in ascidians. Nat Commun 6:8719 (2015). PubMed: 26515371