Anti-Smad1 (phospho S187) 抗体 (ab73211)
Key features and details
- Rabbit polyclonal to Smad1 (phospho S187)
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
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製品名
Anti-Smad1 (phospho S187) antibody
Smad1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Smad1 (phospho S187) -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Mouse, Human -
免疫原
Synthetic peptide corresponding to Human Smad1 (phospho S187).
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, PBS
Without Mg2+ and Ca2+ -
Concentration information loading...
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精製度
Immunogen affinity purified -
特記事項(精製)
ab73211 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab73211の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/500 - 1/1000. Detects a band of approximately 60 kDa (predicted molecular weight: 52 kDa).
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IHC-P |
Use at an assay dependent concentration.
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特記事項 |
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WB
1/500 - 1/1000. Detects a band of approximately 60 kDa (predicted molecular weight: 52 kDa). |
IHC-P
Use at an assay dependent concentration. |
ターゲット情報
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機能
Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD1 is a receptor-regulated SMAD (R-SMAD). SMAD1/OAZ1/PSMB4 complex mediates the degradation of the CREBBP/EP300 repressor SNIP1. -
組織特異性
Ubiquitous. Highest expression seen in the heart and skeletal muscle. -
配列類似性
Belongs to the dwarfin/SMAD family.
Contains 1 MH1 (MAD homology 1) domain.
Contains 1 MH2 (MAD homology 2) domain. -
翻訳後修飾
Phosphorylated on serine by BMP type 1 receptor kinase.
Ubiquitin-mediated proteolysis by SMAD-specific E3 ubiquitin ligase SMURF1. -
細胞内局在
Cytoplasm. Nucleus. Cytoplasmic in the absence of ligand. Migrates to the nucleus when complexed with SMAD4. Co-localizes with LEMD3 at the nucleus inner membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 4086 Human
- Entrez Gene: 17125 Mouse
- Omim: 601595 Human
- SwissProt: Q15797 Human
- SwissProt: P70340 Mouse
- Unigene: 604588 Human
- Unigene: 223717 Mouse
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別名
- BSP-1 antibody
- BSP1 antibody
- HsMAD1 antibody
see all
画像
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All lanes : Anti-Smad1 (phospho S187) antibody (ab73211) at 1/500 dilution
Lane 1 : Mouse muscle cell extract
Lane 2 : Mouse muscle cell extract with immunising phosphopeptide at 10 µg
Lysates/proteins at 30 µg per lane.
Predicted band size: 52 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted? -
Immunohistochemical analysis of Smad1 in paraffin-embedded human breast carcinoma tissue using ab73211 at 1/50 dilution in the presence (right panel) or absence (left panel) of immunising phosphopeptide.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (11)
ab73211 は 11 報の論文で使用されています。
- Ji H et al. CircRNA hsa_circ_0006215 promotes osteogenic differentiation of BMSCs and enhances osteogenesis-angiogenesis coupling by competitively binding to miR-942-5p and regulating RUNX2 and VEGF. Aging (Albany NY) 13:10275-10288 (2021). PubMed: 33819188
- Kushioka J et al. A novel negative regulatory mechanism of Smurf2 in BMP/Smad signaling in bone. Bone Res 8:41 (2020). PubMed: 33298874
- Bridgewater HE et al. The Epstein-Barr Virus-Encoded EBNA1 Protein Activates the Bone Morphogenic Protein (BMP) Signalling Pathway to Promote Carcinoma Cell Migration. Pathogens 9:N/A (2020). PubMed: 32708289
- Ouyang Z et al. LncRNA ENST00000563492 promoting the osteogenesis-angiogenesis coupling process in bone mesenchymal stem cells (BMSCs) by functions as a ceRNA for miR-205-5p. Cell Death Dis 11:486 (2020). PubMed: 32587236
- Li Q et al. Downregulation of miR-98 contributes to hypoxic pulmonary hypertension by targeting ALK1. Mol Med Rep 20:2167-2176 (2019). PubMed: 31322216