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RabMAb

Anti-Smad1 抗体 [EP565Y] (ab33902)

製品の概要

  • 製品名
    Anti-Smad1 antibody [EP565Y]
    Smad1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EP565Y] to Smad1
  • 由来種
    Rabbit
  • アプリケーション
    適用あり: ICC/IF, WBmore details
    適用なし: Flow Cyt,IHC or IP
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    A synthetic peptide corresponding to residues in human Smad1.

  • ポジティブ・コントロール
    • ICC/If: HeLa cells. WB: HeLa cell lysate.
  • 特記事項

    Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab33902 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF 1/100 - 1/250.
WB 1/500 - 1/2000. Detects a band of approximately 58 kDa (predicted molecular weight: 52 kDa).
  • 追加情報
    Is unsuitable for Flow Cyt,IHC or IP.
  • ターゲット情報

    • 機能
      Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD1 is a receptor-regulated SMAD (R-SMAD). SMAD1/OAZ1/PSMB4 complex mediates the degradation of the CREBBP/EP300 repressor SNIP1.
    • 組織特異性
      Ubiquitous. Highest expression seen in the heart and skeletal muscle.
    • 配列類似性
      Belongs to the dwarfin/SMAD family.
      Contains 1 MH1 (MAD homology 1) domain.
      Contains 1 MH2 (MAD homology 2) domain.
    • 翻訳後修飾
      Phosphorylated on serine by BMP type 1 receptor kinase.
      Ubiquitin-mediated proteolysis by SMAD-specific E3 ubiquitin ligase SMURF1.
    • 細胞内局在
      Cytoplasm. Nucleus. Cytoplasmic in the absence of ligand. Migrates to the nucleus when complexed with SMAD4. Co-localizes with LEMD3 at the nucleus inner membrane.
    • Information by UniProt
    • 参照データベース
    • 別名
      • BSP-1 antibody
      • BSP1 antibody
      • HsMAD1 antibody
      • JV4-1 antibody
      • JV41 antibody
      • MAD homolog 1 antibody
      • MAD mothers against decapentaplegic homolog 1 antibody
      • Mad related protein 1 antibody
      • Mad-related protein 1 antibody
      • MADH1 antibody
      • MADR1 antibody
      • Mothers against decapentaplegic homolog 1 antibody
      • Mothers against DPP homolog 1 antibody
      • SMA- AND MAD-RELATED PROTEIN 1 antibody
      • SMAD 1 antibody
      • SMAD family member 1 antibody
      • SMAD mothers against DPP homolog 1 antibody
      • Smad1 antibody
      • SMAD1_HUMAN antibody
      • TGF beta signaling protein 1 antibody
      • Transforming growth factor-beta-signaling protein 1 antibody
      see all

    画像

    • Anti-Smad1 antibody [EP565Y] (ab33902) at 1/500 dilution + HeLa cell lysate

      Predicted band size: 52 kDa
      Observed band size: 58 kDa (why is the actual band size different from the predicted?)

    • Ab33902, at 1/100 dilution, staining Smad1 in HeLa cells by Immunocytochemistry/Immunofluorescence.

    参考文献

    This product has been referenced in:
    • Chang H  et al. Mutant Runx2 regulates amelogenesis and osteogenesis through a miR-185-5p-Dlx2 axis. Cell Death Dis 8:3221 (2017). Read more (PubMed: 29242628) »
    • Dexheimer V  et al. Differential expression of TGF-ß superfamily members and role of Smad1/5/9-signalling in chondral versus endochondral chondrocyte differentiation. Sci Rep 6:36655 (2016). WB ; Human . Read more (PubMed: 27848974) »

    See all 10 Publications for this product

    レビューと Q&A

    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (skin)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: 10mM sodium citrate buffer pH6
    Permeabilization
    No
    Specification
    skin
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
    Fixative
    10% buffered formalin
    Username

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    Verified customer

    投稿 Oct 29 2015

    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (pancreatic tumor)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: 10mM Citrate buffer pH6
    Permeabilization
    No
    Specification
    pancreatic tumor
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
    Fixative
    10% buffered formalin
    Username

    Abcam user community

    Verified customer

    投稿 Oct 29 2015

    Application
    Western blot
    Sample
    Human Cell lysate - nuclear (Normal Human Keratinocyte)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    20 µg
    Specification
    Normal Human Keratinocyte
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Username

    Abcam user community

    Verified customer

    投稿 Apr 15 2015

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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