製品の概要

  • 製品名Anti-SM22 alpha antibody
    SM22 alpha 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal to SM22 alpha
  • アプリケーション適用あり: WB, ELISAmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Recombinant full length Human SM22 alpha protein (AAH04927) with tag.

  • ポジティブ・コントロール
    • SM22 alpha transfected 293T cell lysate. Recombinant tagged Human SM22 alpha protein.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab77442 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 23 kDa (predicted molecular weight: 23 kDa).
ELISA Use at an assay dependent dilution.

ターゲット情報

  • 機能Actin cross-linking/gelling protein (By similarity). Involved in calcium interactions and contractile properties of the cell that may contribute to replicative senescence.
  • 配列類似性Belongs to the calponin family.
    Contains 1 calponin-like repeat.
    Contains 1 CH (calponin-homology) domain.
  • 細胞内局在Cytoplasm.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 22 kDa actin binding protein antibody
    • 22 kDa actin-binding protein antibody
    • Human 22kDa smooth muscle protein antibody
    • Protein WS3-10 antibody
    • SM22 alpha antibody
    • SM22 antibody
    • SM22-alpha antibody
    • SMCC antibody
    • Smooth muscle protein 22-alpha antibody
    • TAGL_HUMAN antibody
    • TAGLN antibody
    • TAGLN1 antibody
    • Transgelin antibody
    • Transgelin variant 2 antibody
    • WS3 10 antibody
    see all

Anti-SM22 alpha antibody 画像

  • All lanes : Anti-SM22 alpha antibody (ab77442) at 1 µg/ml

    Lane 1 : SM22 alpha transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat anti-mouse IgG (H&L)-HRP conjugate at 1/2500 dilution

    Predicted band size : 23 kDa
    Observed band size : 23 kDa
  • Anti-SM22 alpha antibody (ab77442) at 1 µg/ml + Recombinant tagged Human SM22 alpha protein at 0.2 µg

    Secondary
    Goat anti-mouse IgG (H&L)-HRP conjugate at 1/5000 dilution

    Predicted band size : 23 kDa
    Observed band size : 48 kDa (why is the actual band size different from the predicted?)

Anti-SM22 alpha antibody (ab77442) 使用論文

ab77442 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab77442.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"