製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to SM22 alpha
  • 特異性
    Recognises a band at 23kD that is specifically blocked by the immunising peptide.
  • アプリケーション
    適用あり: ICC/IF, IHC-P, IHC-Fr, WB, ICCmore details
  • 種交差性
    交差種: Mouse, Rat, Chicken, Cow, Human, Pig
  • 免疫原

    Synthetic peptide, conjugated to KLH derived from within residues 150 - 200 of Mouse SM22 alpha.

    .

  • ポジティブ・コントロール
    • This antibody gave a positive signal the following lysates for Western Blot: HeLa; HeLa Nuclear; Human Skeletal Muscle; Human Colon; Mouse Colon; Rat Colon ICC/IF: Hela cells

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab14106 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 1 - 5 µg/ml.
IHC-P 1/200.
IHC-Fr Use at an assay dependent concentration.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 23 kDa (predicted molecular weight: 23 kDa).
ICC Use at an assay dependent concentration.

ターゲット情報

Anti-SM22 alpha antibody 画像

  • ab14106 stained Hela cells. The cells were 100% methanol fixed for 5 minutes, permeabilized in 0.1% PBS-Triton X-100 for 5 min and then incubated in 1%BSA / 10% normal Goat serum / 0.3M glycine in 0.1% PBS-Tween for 1 hour at room temperature to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab14106 at 1µg/ml) overnight at +4°C. The secondary antibody (pseudo-colored green) was ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed  used at a 2/1000 dilution for 1hour at room temperature. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1hour at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.

  • ab14106 staining SM22 alpha in mouse muscle cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100 and blocked with 10% serum for 30 minutes at 25°C. Samples were incubated with primary antibody (1/100 in PBS) for 18 hours at 4°C. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG polyclonal was used as the secondary antibody at a dilution of 1/1000.

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  • All lanes : Anti-SM22 alpha antibody (ab14106) at 2 µg/ml

    Lane 1 : HeLa Whole Cell Lysate
    Lane 2 : HeLa Nuclear Extract
    Lane 3 : Human Skeletal Muscle Tissue Lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Alexa Fluor Goat polyclonal to Rabbit IgG at 1/10000 dilution

    Predicted band size : 23 kDa
    Observed band size : 23 kDa

    Lane 1 - 3 : SM22 alpha antibody (ab14106) at 1 ug/ml

    Lane 1 : HeLa Whole Cell Lysate at 20 ug
    Lane 2 : HeLa Nuclear Extract at 20 ug
    Lane 3 : Human Muscle lysate at 20 ug

    Secondary
    Alexa Fluor Goat polyclonal to Rabbit IgG at 1/10000 dilution

    Observed band size : 23kD

    The band was completed abolished by peptide blocking with ab16067 (immunising peptide) - not shown.

     


     

     

     

  • ab14106, at 1/100, staining SM22 alpha in chicken smooth muscle tissue sections by immunohistochemistry (formalin/PFA-fixed paraffin-embedded sections). Sections were methacarn (60% MeOH, 30% choloform, 10% acetic acid) fixed, prior to blocking in 2% serum for 5 minutes at 25°C and then incubated with ab14106, for 1 hour at 25°C. Alexa fluor® 488 goat polyclonal to rabbit Ig, diluted 1/100, was used as the secondary antibody.

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  • All lanes : Anti-SM22 alpha antibody (ab14106) at 1/1000 dilution

    Lane 1 : Lysates prepared from primary Human smooth muscle cells
    Lane 2 : Lysates prepared from primary Human smooth muscle cells
    Lane 3 : Lysates prepared from primary Human smooth muscle cells

    Lysates/proteins at 30 µg per lane.

    Secondary
    HRP-conjugated bovine polyclonal to rabbit IgG at 1/2000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 23 kDa
    Observed band size : 23 kDa


    Exposure time : 2 minutes

    This image is a courtesy of Anonymous Abreview

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  • All lanes : Anti-SM22 alpha antibody (ab14106) at 1 µg/ml

    Lane 1 : Human colon tissue lysate - total protein (ab30051)
    Lane 2 : Colon (Mouse) Tissue Lysate
    Lane 3 : Colon (Rat) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed at 1/50000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 23 kDa
    Observed band size : 23 kDa
    Additional bands at : 115 kDa,20 kDa,36 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 5 seconds

    This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab14106 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406.


  • Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 23 kDa

  • Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 23 kDa


    Exposure time : 30 seconds

Anti-SM22 alpha antibody (ab14106) 使用論文

This product has been referenced in:
  • Nakaya M  et al. Cardiac myofibroblast engulfment of dead cells facilitates recovery after myocardial infarction. J Clin Invest 127:383-401 (2017). ICC, Flow Cyt . Read more (PubMed: 27918308) »
  • Wu Y  et al. MicroRNA-214 regulates smooth muscle cell differentiation from stem cells by targeting RNA-binding protein QKI. Oncotarget 8:19866-19878 (2017). WB . Read more (PubMed: 28186995) »

See all 74 Publications for this product

Product Wall

Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Adult Heart)
Permeabilization
Yes - 0.5% Tween-20 in block
Specification
Adult Heart
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde
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投稿 Apr 03 2017

Application
Western blot
Sample
Cow Tissue lysate - whole (Kidney)
Gel Running Conditions
Reduced Denaturing (14)
Loading amount
20 µg
Specification
Kidney
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 20°C
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投稿 Sep 28 2016

Application
Western blot
Sample
Human Cell lysate - whole cell (vascular smooth muscle cell)
Gel Running Conditions
Reduced Denaturing
Loading amount
20 µg
Specification
vascular smooth muscle cell
Blocking step
Milk as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
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投稿 May 05 2015

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Human arterial myocytes)
Specification
Human arterial myocytes
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 2% · Temperature: 20°C
Fixative
Paraformaldehyde
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投稿 Apr 08 2015

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 25°C
Sample
Mouse Cell (muscle cell)
Specification
muscle cell
Permeabilization
Yes - 0.1%Triton X-100
Fixative
Paraformaldehyde
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投稿 Apr 25 2014

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
PBS, 1% BSA, 10% goat serum, 0.1% TX-100 as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
Sample
Mouse Cell (primary embryonic epicardial)
Specification
primary embryonic epicardial
Permeabilization
Yes - 0.5% Triton X-100
Fixative
Formaldehyde
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投稿 Jan 30 2014

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: RT°C
Sample
Human Cell (Human Artery Smooth Muscle cells)
Specification
Human Artery Smooth Muscle cells
Permeabilization
Yes - 0.1% Triton X-100
Fixative
Paraformaldehyde
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投稿 Jan 16 2014

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C
Sample
Human Cell (Human Umbilical Artery Smooth Muscle Cells)
Specification
Human Umbilical Artery Smooth Muscle Cells
Permeabilization
Yes - 0.1% Triton X-100
Fixative
Paraformaldehyde
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投稿 Dec 09 2013

Application
Western blot
Loading amount
30 µg
Gel Running Conditions
Reduced Denaturing (8% gel)
Sample
Human Cell lysate - whole cell (Human Umbilical Artery Smooth Muscle Cells)
Specification
Human Umbilical Artery Smooth Muscle Cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
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投稿 Oct 07 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Loading amount
30 µg
Gel Running Conditions
Reduced Denaturing (11% SDS-PAGE)
Sample
Human Cell lysate - whole cell (HUVEC cell lysate)
Specification
HUVEC cell lysate
Treatment
5ng/ml TNF-alpha for 24hrs
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
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投稿 Jul 11 2013

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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