製品の概要
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製品名Anti-SLX4 antibody
SLX4 一次抗体 製品一覧 -
製品の詳細Rabbit polyclonal to SLX4
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由来種Rabbit
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アプリケーション適用あり: WBmore details
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種交差性交差種: Human
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免疫原
Synthetic peptide conjugated to KLH derived from within residues 900 - 1000 of Human SLX4.
(Peptide available as ab116498.)
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ポジティブ･コントロール
- This antibody gave a positive signal in HEK293 and U2OS whole cell lysates.
製品の特性
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製品の状態Liquid
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保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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バッファーpH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
精製度Immunogen affinity purified
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ポリ/モノポリクローナル
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アイソタイプIgG
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研究分野
関連製品
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Compatible Secondaries
アプリケーション
Our Abpromise guarantee covers the use of ab100997 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | Use a concentration of 1 µg/ml. Detects a band of approximately 200 kDa (predicted molecular weight: 200 kDa). |
ターゲット情報
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機能Regulatory subunit that interacts with and increases the activity of different structure-specific endonucleases. Has several distinct roles in protecting genome stability by resolving diverse forms of deleterious DNA structures originating from replication and recombination intermediates and from DNA damage. Component of the SLX1-SLX4 structure-specific endonuclease that resolves DNA secondary structures generated during DNA repair and recombination. Has endonuclease activity towards branched DNA substrates, introducing single-strand cuts in duplex DNA close to junctions with ss-DNA. Has a preference for 5'-flap structures, and promotes symmetrical cleavage of static and migrating Holliday junctions (HJs). Resolves HJs by generating two pairs of ligatable, nicked duplex products. Interacts with the structure-specific ERCC4-ERCC1 endonuclease and promotes the cleavage of bubble structures. Interacts with the structure-specific MUS81-EME1 endonuclease and promotes the cleavage of 3'-flap and replication fork-like structures. SLX4 is required for recovery from alkylation-induced DNA damage and is involved in the resolution of DNA double-strand breaks.
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配列類似性Belongs to the SLX4 family.
Contains 1 BTB (POZ) domain. -
翻訳後修飾Phosphorylated upon DNA damage, probably by ATM or ATR.
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細胞内局在Nucleus. Localizes to sites of DNA dammage.
- Information by UniProt
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参照データベース
- Entrez Gene: 84464 Human
- Omim: 613278 Human
- SwissProt: Q8IY92 Human
- Unigene: 143681 Human
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別名
- BTB (POZ) domain containing 12 antibody
- BTB/POZ domain-containing protein 12 antibody
- BTBD12 antibody
see all
画像
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Western blot - Anti-SLX4 antibody (ab100997)All lanes : Anti-SLX4 antibody (ab100997) at 1 µg/ml
Lane 1 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lane 2 : U2OS (Human osteosarcoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 200 kDa
Observed band size: 200 kDa
Additional bands at: 175 kDa (possible isoform), 42 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 10 minutes
This antibody was raised against an immunogen that is predicted to recognize isoforms 1 and 2 of SLX4. The predicted molecular weights of isoforms 1 and 2 are 200 kDa and 166 kDa respectively.
プロトコール
参考文献
ab100997 has not yet been referenced specifically in any publications.
レビューと Q&A
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"