Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Detects a band of approximately 9 kDa (predicted molecular weight: 11 kDa).
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
SLURP1 (Secreted LY6/PLAUR domain containing 1) belongs to the Ly6/uPAR family but lacks a GPI-anchoring signal sequence. SLURP1 potentiates alpha-7 nicotinic acetylcholine receptors (CHRNA7) in keratinocytes and is implicated in maintaining the physiological and structural integrity of the keratinocyte layers of the skin. It is a marker of late differentiation of the skin and has an antitumor activity. Mutations in the SLURP1 gene are the cause of Mal de Meleda (MDM), a rare autosomal recessive genetic disease, characterized by inflammatory palmoplantar keratoderma.
IHC image of SLURP1 staining in Human normal skin formalin fixed paraffin embedded tissue section*, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab93840, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
Western blot - Anti-SLURP1 antibody (ab93840)
Anti-SLURP1 antibody (ab93840) at 1 µg/ml + Human skin tissue lysate - total protein (ab30166) at 10 µg
Secondary Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution
The band observed at 9 kDa could potentially be a cleaved form of SLURP1 due to the presence of a 22 amino acid signal peptide.
This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab93840 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406