製品の概要

  • 製品名
    Anti-SLUG antibody [mAbcam51772]
    SLUG 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [mAbcam51772] to SLUG
  • アプリケーション
    適用あり: Flow Cyt, WB, ICC/IF, IHC-Frmore details
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Chicken, Cow, Pig, Xenopus laevis
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 100 - 200 of Human SLUG.

    (Peptide available as ab27840.)

  • ポジティブ・コントロール
    • Mouse Skin Tissue Lysate
  • 特記事項

    This antibody clone is manufactured by Abcam.

    Alternative versions available:
    Anti-SLUG antibody (FITC) [mAbcam51772] (ab150214)

    If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab51772 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Flow Cyt Use at an assay dependent concentration. PubMed: 24932263ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 30 kDa.
ICC/IF 1/100.
IHC-Fr 1/100.

ターゲット情報

  • 機能
    Transcriptional repressor. Involved in the generation and migration of neural crest cells.
  • 組織特異性
    Expressed in placenta and adult heart, pancreas, liver, kidney and skeletal muscle.
  • 関連疾患
    Defects in SNAI2 are the cause of Waardenburg syndrome type 2D (WS2D) [MIM:608890]. WS2 is a genetically heterogeneous, autosomal dominant disorder characterized by sensorineural deafness, pigmentary disturbances, and absence of dystopia canthorum. The frequency of deafness is higher in WS2 than in WS1.
  • 配列類似性
    Belongs to the snail C2H2-type zinc-finger protein family.
    Contains 5 C2H2-type zinc fingers.
  • 細胞内局在
    Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • MGC10182 antibody
    • Neural crest transcription factor Slug antibody
    • Protein snail homolog 2 antibody
    • Slug (chicken homolog) zinc finger protein antibody
    • Slug homolog zinc finger protein antibody
    • Slug zinc finger protein antibody
    • SLUGH antibody
    • SLUGH 1 antibody
    • SLUGH1 antibody
    • SNAI 2 antibody
    • Snai2 antibody
    • SNAI2_HUMAN antibody
    • Snail 2 antibody
    • Snail homolog 2 antibody
    • Snail2 antibody
    • WS 2D antibody
    • WS2D antibody
    • Zinc finger protein SLUG antibody
    • Zinc finger protein SNAI2 antibody
    see all

画像

  • All lanes : Anti-SLUG antibody [mAbcam51772] (ab51772)

    Lane 1 : Untransfected MCF7 cells
    Lane 2 : MCF7 cells transfected with SLUG
    Lane 3 : MCF7 cells transfected with Snail


    Predicted band size : 30 kDa
    Observed band size : 30 kDa
  • Anti-SLUG antibody [mAbcam51772] (ab51772) at 5 µg/ml + Mouse skin tissue lysate - total protein (ab4025) at 10 µg

    Secondary
    Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size : 30 kDa
    Observed band size : 30 kDa
    Additional bands at : 34 kDa,60 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 10 minutes
  • All lanes : Anti-SLUG antibody [mAbcam51772] (ab51772) at 1 µg/ml

    Lane 1 : Skin tissue lysate from Slug heterozygous mouse
    Lane 2 : Skin tissue lysate from Slug knockout mouse


    Performed under reducing conditions.

    Predicted band size : 30 kDa
  • ab51772 staining SLUG in mouse mammary gland cells by Immunocytochemistry/ Immunofluorescence. Cells were fixed with paraformaldehyde, permeabilized with PF4% Triton 0,5% for 5min and blocking with 10% serum for 1 hour at RT was performed. Samples were incubated with primary antibody (1/100) for 1 hour. An Alexa Fluor®488-conjugated goat polyclonal to mouse IgG (H+L) was used at dilution at 1/750 as secondary antibody. Blue colour in the image represents DAPI staining.

    See Abreview

参考文献

This product has been referenced in:
  • Kudinov AE  et al. Musashi-2 (MSI2) supports TGF-ß signaling and inhibits claudins to promote non-small cell lung cancer (NSCLC) metastasis. Proc Natl Acad Sci U S A 113:6955-60 (2016). WB . Read more (PubMed: 27274057) »
  • Sun Y  et al. Slug overexpression induces stemness and promotes hepatocellular carcinoma cell invasion and metastasis. Oncol Lett 7:1936-1940 (2014). WB, Flow Cyt ; Human . Read more (PubMed: 24932263) »

See all 4 Publications for this product

レビューと Q&A

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (breast cancer tissue, tonsil)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: sodium citrate pH 6
Permeabilization
Yes - tween 20
Specification
breast cancer tissue, tonsil
Blocking step
ab diluent as blocking agent for 10 minute(s) · Concentration: 100% · Temperature: 25°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

投稿 Mar 07 2017

Application
Western blot
Sample
Human Tissue lysate - whole (Human prostate cancer cell)
Gel Running Conditions
Reduced Denaturing (12%)
Loading amount
30 µg
Specification
Human prostate cancer cell
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Aug 31 2015

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
Sample
Human Cell (endothelial cell)
Specification
endothelial cell
Permeabilization
No
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

投稿 Jul 29 2014

Application
Western blot
Sample
Apteronotus leptorhynchus Tissue lysate - whole (Brain)
Loading amount
50 µg
Specification
Brain
Gel Running Conditions
Reduced Denaturing (4-15%)
Blocking step
Milk as blocking agent for 15 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Abcam user community

Verified customer

投稿 Jan 23 2013

Thank you for your enquiry.

I have attached the Certificate of Compliance you requested. Please let me know if you need anything further.

Gracias por contactarnos. Te pido disculpas por la espera, he contactado con mis compañeros del laboratorio para poder darte una información más completa.

La secuencia de aminoácidos del inmunógeno de ab5177...

Read More
Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Apteronotus leptorhynchus Tissue sections (Brain)
Specification
Brain
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.3% Triton X-100
Blocking step
3% sheep serum, 1% BSA, 1% teleostean gelatine in TBS as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 24°C
Username

Dr. Ruxandra Sirbulescu

Verified customer

投稿 Nov 14 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (skin)
Specification
skin
Fixative
Paraformaldehyde
Permeabilization
No
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
Username

Ms. Mayssaa NASSOUR

Verified customer

投稿 Jul 27 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (mouse mammary gland)
Specification
mouse mammary gland
Fixative
Paraformaldehyde
Permeabilization
Yes - PF4% triton 0,5% 5min
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
Username

Ms. Mayssaa NASSOUR

Verified customer

投稿 Jul 22 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Tissue lysate - whole (skin, comma D ß cell line)
Loading amount
40 µg
Specification
skin, comma D ß cell line
Gel Running Conditions
Reduced Denaturing (12%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Ms. Mayssaa NASSOUR

Verified customer

投稿 Jul 21 2009

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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