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Synthetic peptide conjugated to KLH derived from within residues 100 - 200 of Human SLUG.
(Peptide available as ab27840.)
This antibody clone is manufactured by Abcam.
Our Abpromise guarantee covers the use of ab51772 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use at an assay dependent concentration. PubMed: 24932263ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.|
|WB||Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 30 kDa.|
ab51772 staining SLUG in mouse mammary gland cells by Immunocytochemistry/ Immunofluorescence. Cells were fixed with paraformaldehyde, permeabilized with PF4% Triton 0,5% for 5min and blocking with 10% serum for 1 hour at RT was performed. Samples were incubated with primary antibody (1/100) for 1 hour. An Alexa Fluor®488-conjugated goat polyclonal to mouse IgG (H+L) was used at dilution at 1/750 as secondary antibody. Blue colour in the image represents DAPI staining.