製品の概要

  • 製品名Anti-SLUG antibody
    SLUG 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to SLUG
  • 特異性ab27568 detects the overexpressing human Slug protein, however we have no data on the detection of the endogenous Human protein with this current batch. ab27568 does not cross react with overexpressed SNAIL protein in cell lysates.
  • アプリケーション適用あり: WB, IHC-P, IHC-Fr, ICC/IFmore details
  • 種交差性
    交差種: Mouse, Rat, Chicken
    交差が予測される動物種: Cow, Dog, Human, Pig, Xenopus laevis
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 100 to the C-terminus of Human SLUG.

  • ポジティブ・コントロール
    • This antibody gave a positive signal in the following tissue lysates: Mouse heart, Rat heart as well as MCF7 cell lysate overexpressing SLUG protein.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab27568 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/1000. Detects a band of approximately 30-34 kDa (predicted molecular weight: 30 kDa).
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
IHC-Fr Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration. PubMed: 18716062

ターゲット情報

  • 機能Transcriptional repressor. Involved in the generation and migration of neural crest cells.
  • 組織特異性Expressed in placenta and adult heart, pancreas, liver, kidney and skeletal muscle.
  • 関連疾患Defects in SNAI2 are the cause of Waardenburg syndrome type 2D (WS2D) [MIM:608890]. WS2 is a genetically heterogeneous, autosomal dominant disorder characterized by sensorineural deafness, pigmentary disturbances, and absence of dystopia canthorum. The frequency of deafness is higher in WS2 than in WS1.
  • 配列類似性Belongs to the snail C2H2-type zinc-finger protein family.
    Contains 5 C2H2-type zinc fingers.
  • 細胞内局在Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • MGC10182 antibody
    • Neural crest transcription factor Slug antibody
    • Protein snail homolog 2 antibody
    • Slug (chicken homolog) zinc finger protein antibody
    • Slug homolog zinc finger protein antibody
    • Slug zinc finger protein antibody
    • SLUGH antibody
    • SLUGH 1 antibody
    • SLUGH1 antibody
    • SNAI 2 antibody
    • Snai2 antibody
    • SNAI2_HUMAN antibody
    • Snail 2 antibody
    • Snail homolog 2 antibody
    • Snail2 antibody
    • WS 2D antibody
    • WS2D antibody
    • Zinc finger protein SLUG antibody
    • Zinc finger protein SNAI2 antibody
    see all

Anti-SLUG antibody 画像

  • Transverse section showing Slug-expressing neural crest cells (green) in the dorsal neural tube (NT) in the hindbrain of a quail embryo. 4% PFA fixed embryonic day 1.7 Japanese Quail embryo, frozen in Tissutek, sectioned 12 um. Ab27568 1/400, incubated overnight. Goat anti-rabbit: Alexa488 (Molecular Probes) 1/1000 incubated for 2 hours.
  • All lanes : Anti-SLUG antibody (ab27568) at 1 µg/ml

    Lane 1 : Heart (Mouse) Tissue Lysate
    Lane 2 : Heart (Rat) Tissue Lysate

    Lysates/proteins at 30 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Predicted band size : 30 kDa
    Observed band size : 30 kDa

    Secondary antibody - goat anti-rabbit HRP (preadsorbed)

  • Image courtesy of Human Protein Atlas

    ab27568 staining in human placenta, showing staining of the trophoblastic cells (in brown). Paraffin embedded placental tissue was incubated with ab27568 (1:750 dilution) for 30 mins at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH 6. ab27568 was tested in a tissue microarray (TMA) containing a wide range of normal and cancer tissues as well as a cell microarray consisting of a range of commonly used, well characterised human cell lines. Further results for this antibody can be found at www.proteinatlas.org

    .

  • All lanes : Anti-SLUG antibody (ab27568) at 1/1000 dilution

    Lane 1 : MCF7 cell lysate overexpressing SLUG protein
    Lane 2 : MCF7 lysate overexpressing SNAIL protein
    Lane 3 : MCF7 lysate tranfected with vector control plasmid

    Secondary
    HRP-conjugated anti-rabbit antibody at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size : 30 kDa
    Observed band size : 34 kDa (why is the actual band size different from the predicted?)

    This image is courtesy of Archana Dhasarathy, Ph.D., NC

    ab27568 detects a band of 34 kDa in MCF7 cells that have been transfected with a vector that overexpresses the SLUG protein. This antibody does not crossreact with the SNAIL protein, as it did not recognize this protein in MCF7 cells overexpressing SNAIL. ab27568 was unable to detect endogenous SLUG in MDA-MB231 cells (not shown).
  • ab27568 staining mouse embryonic stem cells by ICC/IF.  Cells were formaldehyde fixed and permeabilized in 0.1% Triton X-100 prior to blocking with 0.1% BSA and 1% serum for 30 minutes at 21°C.  The primary antibody was diluted 1/100 and incubated for 2 hours at 21°C.  An Alexa Fluor® 488 conjugated goat anti-rabbit antibody, diluted 1/500, was used as the secondary.

    See Abreview

Anti-SLUG antibody (ab27568) 使用論文

This product has been referenced in:
  • Gu A  et al. Slug Is Associated With Tumor Metastasis and Angiogenesis in Ovarian Cancer. Reprod Sci N/A:N/A (2016). IHC ; Human . Read more (PubMed: 27368877) »
  • Gao Y  et al. The dual function of PRMT1 in modulating epithelial-mesenchymal transition and cellular senescence in breast cancer cells through regulation of ZEB1. Sci Rep 6:19874 (2016). WB ; Human . Read more (PubMed: 26813495) »

See all 26 Publications for this product

Product Wall

Application IHC - Wholemount
Sample Zebrafish Embryo (wholemount)
Specification wholemount
Username

Abcam user community

Verified customer

投稿 Dec 04 2013

Application Immunocytochemistry/ Immunofluorescence
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10%
Sample Human Cell (mammalian cancer cells)
Specification mammalian cancer cells
Permeabilization Yes - Tween20
Fixative Formaldehyde
Username

Mr. Gabriel Ortega

Verified customer

投稿 Jul 01 2013

Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (E10.5 mouse embryo sections)
Specification E10.5 mouse embryo sections
Fixative Paraformaldehyde
Permeabilization Yes - PBST (PBS / 0.5% v/v TritonX100 (Sigma)
Blocking step NOVOCASTRA protein block as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Oct 11 2012

Thank you for contacting Abcam regarding ab27568.


I am sorry to hear that a customer is experiencing difficulties with this antibody in IHC. Thank you for passing along the protocol information. Overall this looks pretty standard, and bas...

Read More

Gracias por contactarnos. Te pido disculpas por la espera, he contactado con mis compañeros del laboratorio para poder darte una información más completa.

La secuencia de aminoácidos del inmunógeno de ab5177...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Lung adenocarcinoma induced by urethane)
Specification Lung adenocarcinoma induced by urethane
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate pH 6
Permeabilization No
Blocking step Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Oct 08 2010

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (heart, pancreas)
Specification heart, pancreas
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrate
Permeabilization No
Blocking step casein based (Biocare) as blocking agent for 10 minute(s) · Concentration: 00% · Temperature: 25°C
Username

Mrs. Julia Abramowitz

Verified customer

投稿 Nov 04 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (skin, comma D ß cell line)
Loading amount 40 µg
Specification skin, comma D ß cell line
Gel Running Conditions Reduced Denaturing (12)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Ms. Mayssaa NASSOUR

Verified customer

投稿 Jul 10 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Chicken Cell (Embryonic section of spinal cord, stage 10-11)
Specification Embryonic section of spinal cord, stage 10-11
Fixative Paraformaldehyde
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1%
Username

Dr. James Briscoe

Verified customer

投稿 Nov 10 2008

Application Western blot
Sample Human Cell lysate - whole cell (smooth muscle cells)
Loading amount 20 µg
Specification smooth muscle cells
Gel Running Conditions Reduced Denaturing (4-12% Bis-Tris)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

投稿 Jul 24 2008

1-10 of 14 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"