Anti-SLP76 (phospho Y145) 抗体 [EP2853Y] (ab75829)


  • 製品名Anti-SLP76 (phospho Y145) antibody [EP2853Y]
    SLP76 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EP2853Y] to SLP76 (phospho Y145)
  • 特異性This antibody detects SLP76 phosphorylated on tyrosine 145.
  • アプリケーション適用あり: ICC/IF, WB, Flow Cytmore details
    適用なし: IHC-P
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide within Human SLP76 aa 100-200 (phospho Y145). The exact sequence is proprietary.
    Database link: Q13094

  • ポジティブ・コントロール
    • Jurkat cell lysates treated with pervanadate.
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.


  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファーPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
  • 精製度Tissue culture supernatant
  • ポリ/モノモノクローナル
  • クローン名EP2853Y
  • アイソタイプIgG
  • 研究分野



Our Abpromise guarantee covers the use of ab75829 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF 1/500.
WB 1/1000 - 1/10000. Predicted molecular weight: 60 kDa.
Flow Cyt 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

  • 追加情報Is unsuitable for IHC-P.
  • ターゲット情報

    • 機能Involved in T-cell antigen receptor mediated signaling.
    • 組織特異性Highly expressed in spleen, thymus and peripheral blood leukocytes. Highly expressed also in T-cell and monocytic cell lines, expressed at lower level in B-cell lines. Not detected in fibroblast or neuroblastoma cell lines.
    • 配列類似性Contains 1 SAM (sterile alpha motif) domain.
      Contains 1 SH2 domain.
    • ドメインThe SH2 domain mediates interaction with SHB.
    • 翻訳後修飾Phosphorylated after T-cell receptor activation by ZAP-70.
    • 細胞内局在Cytoplasm.
    • Information by UniProt
    • 参照データベース
    • 別名
      • 76 kDa tyrosine phosphoprotein antibody
      • CG8697 antibody
      • LCP 2 antibody
      • LCP2 antibody
      • LCP2_HUMAN antibody
      • Lymphocyte cytosolic protein 2 antibody
      • SH2 domain containing leukocyte protein 76 KD antibody
      • SH2 domain containing leukocyte protein of 76kD antibody
      • SH2 domain containing leukocyte protein of 76kDa antibody
      • SH2 domain-containing leukocyte protein of 76 kDa antibody
      • SLP 76 antibody
      • SLP 76 tyrosine phosphoprotein antibody
      • SLP-76 antibody
      • SLP-76 tyrosine phosphoprotein antibody
      • SLP76 antibody
      • SLP76 tyrosine phosphoprotein antibody
      see all

    Anti-SLP76 (phospho Y145) antibody [EP2853Y] 画像

    • ab75829 staining SLP76 (phospho Y145) antibody in both treated (50mM of pervanadate for 5 minutes) and untreated Jurkat (human acute T cell leukemia) cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% Paraformaldehyde and permeabilized with 0.1% Triton X-100. Samples were incubated with primary antibody at a dilution of 1/500. A goat anti rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a dilution of 1/1000.

      DAPI was used as a nuclear counterstain.

    • All lanes : Anti-SLP76 (phospho Y145) antibody [EP2853Y] (ab75829) at 1/200000 dilution

      Lane 1 : Jurkat cell lysates un-treated
      Lane 2 : Jurkat cell lysates treated with pervanadate

      Lysates/proteins at 10 µg per lane.

      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 60 kDa
      Observed band size : 76 kDa (why is the actual band size different from the predicted?)
    • Dot blot analysis of SLP76 (phospho Y145) phospho peptide (Lane 1), SLP76 Non-phospho peptide (Lane 2), labelling SLP76 (phospho Y145) with ab75829 at a dilution of 1/1000. Peroxidase conjugated goat anti-rabbit IgG (H+L)) was used as the secondary antibody at a dilution of 1/2500.

      Blocking and diluting buffer: 5% NFDM/TBST.

      Exposure time: 3 minutes.

    • Flow cytometric analysis of permeabilized Jurkat cells, untreated (green) or pervanadate treated (red) using ab75829 at a 1/100 dilution. Also, pervanadate Jurkat cells using the same antibody preincubated with phospho SLP76 peptide (blue) or non phospho SLP76 peptide (orange).

    Anti-SLP76 (phospho Y145) antibody [EP2853Y] (ab75829) 使用論文

    This product has been referenced in:
    • Yang CY  et al. Dual-specificity phosphatase 14 (DUSP14/MKP6) negatively regulates TCR signaling by inhibiting TAB1 activation. J Immunol 192:1547-57 (2014). Read more (PubMed: 24403530) »
    • Gill T & Levine AD Mitochondria-derived hydrogen peroxide selectively enhances T cell receptor-initiated signal transduction. J Biol Chem 288:26246-55 (2013). Read more (PubMed: 23880762) »

    See all 2 Publications for this product

    Product Wall

    Application Western blot
    Loading amount 100000 cells
    Gel Running Conditions Reduced Denaturing (10%)
    Sample Human Cell lysate - whole cell (jurkats)
    Specification jurkats
    Treatment TCR activation with OKT3 and anti CD8
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C

    Dr. Tejpal Gill

    Verified customer

    投稿 May 13 2014

    Thank you for contacting us.

    Your credit note ID is 23976.

    I am sorry that this antibody did not perform as stated on the datasheet, I have asked our Finance department to issue a credit note for you. The credit note may be used i...

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    Thank you for your enquiry regarding ab75829 and for taking the time to provide some useful details of the experiments. I am very sorry to hear that you are having problems with this antibody.

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    Thank you for your inquiry.

    The dilution factors for each application listed on the product datasheet for ab75829 are what we currently recommended, it is still relevant.

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