Anti-SLP76 (phospho Y113) 抗体 [EPR2851] (ab75990)

製品の概要

  • 製品名Anti-SLP76 (phospho Y113) antibody [EPR2851]
    SLP76 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EPR2851] to SLP76 (phospho Y113)
  • 特異性ab75990 detects SLP76 phosphorylated on tyrosine 113
  • アプリケーション適用あり: WB, Flow Cytmore details
    適用なし: ICC,IHC-P or IP
  • 種交差性
    交差種: Rabbit, Human
  • 免疫原

    A phospho specific peptide corresponding to residues surrounding tyrosine 113 of human SLP76.

  • ポジティブ・コントロール
    • Jurkat cytoplasmic lysate treated with pervanadate.
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファーPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
  • 精製度Tissue culture supernatant
  • ポリ/モノモノクローナル
  • クローン名EPR2851
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab75990 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/5000 - 1/10000. Detects a band of approximately 76 kDa (predicted molecular weight: 60 kDa).
Flow Cyt 1/10.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

  • 追加情報Is unsuitable for ICC,IHC-P or IP.
  • ターゲット情報

    • 機能Involved in T-cell antigen receptor mediated signaling.
    • 組織特異性Highly expressed in spleen, thymus and peripheral blood leukocytes. Highly expressed also in T-cell and monocytic cell lines, expressed at lower level in B-cell lines. Not detected in fibroblast or neuroblastoma cell lines.
    • 配列類似性Contains 1 SAM (sterile alpha motif) domain.
      Contains 1 SH2 domain.
    • ドメインThe SH2 domain mediates interaction with SHB.
    • 翻訳後修飾Phosphorylated after T-cell receptor activation by ZAP-70.
    • 細胞内局在Cytoplasm.
    • Information by UniProt
    • 参照データベース
    • 別名
      • 76 kDa tyrosine phosphoprotein antibody
      • CG8697 antibody
      • LCP 2 antibody
      • LCP2 antibody
      • LCP2_HUMAN antibody
      • Lymphocyte cytosolic protein 2 antibody
      • SH2 domain containing leukocyte protein 76 KD antibody
      • SH2 domain containing leukocyte protein of 76kD antibody
      • SH2 domain containing leukocyte protein of 76kDa antibody
      • SH2 domain-containing leukocyte protein of 76 kDa antibody
      • SLP 76 antibody
      • SLP 76 tyrosine phosphoprotein antibody
      • SLP-76 antibody
      • SLP-76 tyrosine phosphoprotein antibody
      • SLP76 antibody
      • SLP76 tyrosine phosphoprotein antibody
      see all

    Anti-SLP76 (phospho Y113) antibody [EPR2851] 画像

    • All lanes : Anti-SLP76 (phospho Y113) antibody [EPR2851] (ab75990) at 1/10000 dilution

      Lane 1 : Jurkat cell lysate - untreated
      Lane 2 : Jurkat cell lysate - treated with pervanadate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 60 kDa
      Observed band size : 76 kDa (why is the actual band size different from the predicted?)

    Anti-SLP76 (phospho Y113) antibody [EPR2851] (ab75990) 使用論文

    This product has been referenced in:
    • Cimo AM  et al. CD25 and CD69 induction by a4ß1 outside-in signalling requires TCR early signalling complex proteins. Biochem J 454:109-21 (2013). WB ; Human . Read more (PubMed: 23758320) »
    • Köhler K  et al. Matched sizes of activating and inhibitory receptor/ligand pairs are required for optimal signal integration by human natural killer cells. PLoS One 5:e15374 (2010). WB ; Human . Read more (PubMed: 21179506) »

    See all 2 Publications for this product

    Product Wall

    Application Western blot
    Loading amount 1e+006 cells
    Gel Running Conditions Reduced Denaturing
    Sample Rabbit Cell lysate - whole cell (T cells)
    Specification T cells
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Username

    Dr. youg thaker

    Verified customer

    投稿 May 15 2014

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"