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RabMAb

Anti-SLP76 抗体 [EPR2549(2)] (ab109254)

製品の概要

  • 製品名
    Anti-SLP76 antibody [EPR2549(2)]
    SLP76 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EPR2549(2)] to SLP76
  • アプリケーション
    適用あり: WB, IHC-P, ICCmore details
    適用なし: Flow Cyt or IP
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    A synthetic peptide corresponding to residues near the C-terminus of Human SLP76.

  • ポジティブ・コントロール
    • MOLT4 or HuT78 cell lysates; Human tonsil tissue.
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • バッファー
    PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
  • 精製度
    Tissue culture supernatant
  • ポリ/モノ
    モノクローナル
  • クローン名
    EPR2549(2)
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab109254 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000 - 1/10000. Detects a band of approximately 76 kDa (predicted molecular weight: 60 kDa).
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC 1/100 - 1/250.
  • 追加情報
    Is unsuitable for Flow Cyt or IP.
  • ターゲット情報

    • 機能
      Involved in T-cell antigen receptor mediated signaling.
    • 組織特異性
      Highly expressed in spleen, thymus and peripheral blood leukocytes. Highly expressed also in T-cell and monocytic cell lines, expressed at lower level in B-cell lines. Not detected in fibroblast or neuroblastoma cell lines.
    • 配列類似性
      Contains 1 SAM (sterile alpha motif) domain.
      Contains 1 SH2 domain.
    • ドメイン
      The SH2 domain mediates interaction with SHB.
    • 翻訳後修飾
      Phosphorylated after T-cell receptor activation by ZAP-70.
    • 細胞内局在
      Cytoplasm.
    • Information by UniProt
    • 参照データベース
    • 別名
      • 76 kDa tyrosine phosphoprotein antibody
      • CG8697 antibody
      • LCP 2 antibody
      • LCP2 antibody
      • LCP2_HUMAN antibody
      • Lymphocyte cytosolic protein 2 antibody
      • SH2 domain containing leukocyte protein 76 KD antibody
      • SH2 domain containing leukocyte protein of 76kD antibody
      • SH2 domain containing leukocyte protein of 76kDa antibody
      • SH2 domain-containing leukocyte protein of 76 kDa antibody
      • SLP 76 antibody
      • SLP 76 tyrosine phosphoprotein antibody
      • SLP-76 antibody
      • SLP-76 tyrosine phosphoprotein antibody
      • SLP76 antibody
      • SLP76 tyrosine phosphoprotein antibody
      see all

    画像

    • ab109254 at 1/100 dilution, staining SLP76 in Human tonsil, by Immunohistochemistry, Paraffin-embedded tissue.
    • All lanes : Anti-SLP76 antibody [EPR2549(2)] (ab109254) at 1/1000 dilution

      Lane 1 : HuT78 cell lysate
      Lane 2 : MOLT4 cell lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 60 kDa
    • ab109254 showing positive staining in Normal colon tissue.

    • ab109254 showing negative staining in Normal brain tissue.

    • ab109254 showing negative staining in Normal heart tissue.

    • ab109254 showing positive staining in Normal spleen tissue.

    参考文献

    ab109254 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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