製品の概要

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファーPreservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris buffered saline. pH 7.3
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab17029 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 75 kDa (predicted molecular weight: 61 kDa).

ターゲット情報

  • 機能Involved in T-cell antigen receptor mediated signaling.
  • 組織特異性Highly expressed in spleen, thymus and peripheral blood leukocytes. Highly expressed also in T-cell and monocytic cell lines, expressed at lower level in B-cell lines. Not detected in fibroblast or neuroblastoma cell lines.
  • 配列類似性Contains 1 SAM (sterile alpha motif) domain.
    Contains 1 SH2 domain.
  • ドメインThe SH2 domain mediates interaction with SHB.
  • 翻訳後修飾Phosphorylated after T-cell receptor activation by ZAP-70.
  • 細胞内局在Cytoplasm.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 76 kDa tyrosine phosphoprotein antibody
    • CG8697 antibody
    • LCP 2 antibody
    • LCP2 antibody
    • LCP2_HUMAN antibody
    • Lymphocyte cytosolic protein 2 antibody
    • SH2 domain containing leukocyte protein 76 KD antibody
    • SH2 domain containing leukocyte protein of 76kD antibody
    • SH2 domain containing leukocyte protein of 76kDa antibody
    • SH2 domain-containing leukocyte protein of 76 kDa antibody
    • SLP 76 antibody
    • SLP 76 tyrosine phosphoprotein antibody
    • SLP-76 antibody
    • SLP-76 tyrosine phosphoprotein antibody
    • SLP76 antibody
    • SLP76 tyrosine phosphoprotein antibody
    see all

Anti-SLP76 antibody 画像

  • Anti-SLP76 antibody (ab17029) at 0.3 µg/ml + Jurkat lysate (protein in RIPA buffer) at 35 µg

    Predicted band size : 61 kDa
    Observed band size : 80 kDa (why is the actual band size different from the predicted?)

    Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-SLP76 antibody (ab17029) 使用論文

ab17029 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Sample Goat Cell lysate - whole cell (Jurkat)
Loading amount 1e+006 cells
Specification Jurkat
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 5% · Temperature: 20°C
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投稿 Dec 04 2007

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"