The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 95 kDa.
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Suppresses neurite outgrowth.
Expressed predominantly in the cerebral cortex of the brain but also at low levels in the spinal cord and medulla. Also expressed in some astrocytic brain tumors such as astrocytomas, oligodendrogliomas, glioblastomas, gangliogliomas and primitive neuroectodermal tumors.
Belongs to the SLITRK family. Contains 12 LRR (leucine-rich) repeats. Contains 2 LRRCT domains. Contains 1 LRRNT domain.
ab115594, at 5 µg/ml, staining SLITRK2 in formalin fixed, paraffin embedded Human brain cerebellum by Immunohistochemistry. After incubation with the primary antibody, slides were incubated with biotinylated goat anti-rabbit secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen.
Western blot - Anti-SLITRK2 antibody (ab115594)
Lane 1 : Anti-SLITRK2 antibody (ab115594) at 1 µg/ml Lane 2 : Anti-SLITRK2 antibody (ab115594) at 2 µg/ml
Lane 1 : Rat brain tissue lysate Lane 2 : Rat brain tissue lysate