Anti-Slit1 抗体 (ab88491)


  • 製品名
  • 製品の詳細
    Rabbit polyclonal to Slit1
  • 由来種
  • アプリケーション
    適用あり: ICC/IF, WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Rabbit, Gorilla, Chinese hamster
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 650 - 750 of Human Slit1.

  • ポジティブ・コントロール
    • This antibody gave a positive signal in HEK293 expressing mSlit1-myc whole cell lysate.


  • 製品の状態
  • 保存方法
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー
    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS
    Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • ポリ/モノ
  • アイソタイプ
  • 研究分野


Our Abpromise guarantee covers the use of ab88491 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 5 µg/ml.
WB 1/200. Detects a band of approximately 260 kDa (predicted molecular weight: 168 kDa). Collaborator Data


  • 機能
    Thought to act as molecular guidance cue in cellular migration, and function appears to be mediated by interaction with roundabout homolog receptors. During neural development involved in axonal navigation at the ventral midline of the neural tube and projection of axons to different regions (By similarity). SLIT1 and SLIT2 together seem to be essential for midline guidance in the forebrain by acting as repulsive signal preventing inappropriate midline crossing by axons projecting from the olfactory bulb.
  • 組織特異性
    Predominantly expressed in adult forebrain. Expressed in fetal brain, lung and kidney.
  • 配列類似性
    Contains 1 CTCK (C-terminal cystine knot-like) domain.
    Contains 9 EGF-like domains.
    Contains 1 laminin G-like domain.
    Contains 20 LRR (leucine-rich) repeats.
    Contains 4 LRRCT domains.
    Contains 4 LRRNT domains.
  • 細胞内局在
  • Information by UniProt
  • 参照データベース
  • 別名
    • KIAA0813 antibody
    • MEGF 4 antibody
    • MEGF4 antibody
    • MGC164811 antibody
    • Multiple EGF-like domains protein 4 antibody
    • Multiple epidermal growth factor like domains 4 antibody
    • Multiple epidermal growth factor-like domains protein 4 antibody
    • OTTHUMP00000059392 antibody
    • SLIL 1 antibody
    • SLIL1 antibody
    • Slit 1 antibody
    • SLIT 3 antibody
    • Slit homolog 1 antibody
    • Slit homolog 1 protein antibody
    • Slit-1 antibody
    • Slit1 antibody
    • SLIT1_HUMAN antibody
    • SLIT3 antibody
    see all


  • All lanes : Anti-Slit1 antibody (ab88491) at 1/200 dilution

    Lane 1 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
    Lane 2 : HEK293 cells expressing mSlit1-myc
    Lane 3 : HEK293 cells expressing hSlit2-myc
    Lane 4 : HEK293 cells expressing hSlit3-myc

    Lysates/proteins at 30 µg per lane.

    All lanes : HRP Anti-Rabbit at 1/20000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 168 kDa
    Observed band size: 260 kDa (why is the actual band size different from the predicted?)
    Additional bands at: 90 kDa. We are unsure as to the identity of these extra bands.

    Exposure time: 15 minutes

    Slit1 contains an extensive number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted. Abcam recommends using milk as the blocking agent. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.
  • ICC/IF image of ab88491 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab88491, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.


ab88491 has not yet been referenced specifically in any publications.

レビューと Q&A

Merci beaucoup pour votre appel de hier.

Je suis heureuse que mes collègues du business développement ont pu nous donner la réponse à votre enquête. L'anticorps envoyé en test au group x estune production de l'ab88491. Vous pouvez donc comman...

Read More
Abcam has not validated the combination of species/application used in this Abreview.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Mouse Tissue sections (Embryonic spinal cord)
Embryonic spinal cord
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C

Mr. Carl Hobbs

Verified customer

投稿 Mar 02 2012