Anti-Slit1 抗体 (ab88491)

製品の概要

  • 製品名Anti-Slit1 antibody
    Slit1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Slit1
  • アプリケーション適用あり: ICC/IF, WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Rabbit, Gorilla, Chinese Hamster
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 650 - 750 of Human Slit1.

  • ポジティブ・コントロール
    • This antibody gave a positive signal in HEK293 expressing mSlit1-myc whole cell lysate.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファーpH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS
    Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab88491 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 5 µg/ml.
WB 1/200. Detects a band of approximately 260 kDa (predicted molecular weight: 168 kDa). Collaborator Data

ターゲット情報

  • 機能Thought to act as molecular guidance cue in cellular migration, and function appears to be mediated by interaction with roundabout homolog receptors. During neural development involved in axonal navigation at the ventral midline of the neural tube and projection of axons to different regions (By similarity). SLIT1 and SLIT2 together seem to be essential for midline guidance in the forebrain by acting as repulsive signal preventing inappropriate midline crossing by axons projecting from the olfactory bulb.
  • 組織特異性Predominantly expressed in adult forebrain. Expressed in fetal brain, lung and kidney.
  • 配列類似性Contains 1 CTCK (C-terminal cystine knot-like) domain.
    Contains 9 EGF-like domains.
    Contains 1 laminin G-like domain.
    Contains 20 LRR (leucine-rich) repeats.
    Contains 4 LRRCT domains.
    Contains 4 LRRNT domains.
  • 細胞内局在Secreted.
  • Information by UniProt
  • 参照データベース
  • 別名
    • KIAA0813 antibody
    • MEGF 4 antibody
    • MEGF4 antibody
    • MGC164811 antibody
    • Multiple EGF-like domains protein 4 antibody
    • Multiple epidermal growth factor like domains 4 antibody
    • Multiple epidermal growth factor-like domains protein 4 antibody
    • OTTHUMP00000059392 antibody
    • SLIL 1 antibody
    • SLIL1 antibody
    • Slit 1 antibody
    • SLIT 3 antibody
    • Slit homolog 1 antibody
    • Slit homolog 1 protein antibody
    • Slit-1 antibody
    • Slit1 antibody
    • SLIT1_HUMAN antibody
    • SLIT3 antibody
    see all

Anti-Slit1 antibody 画像

  • All lanes : Anti-Slit1 antibody (ab88491) at 1/200 dilution

    Lane 1 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
    Lane 2 : HEK293 cells expressing mSlit1-myc
    Lane 3 : HEK293 cells expressing hSlit2-myc
    Lane 4 : HEK293 cells expressing hSlit3-myc

    Lysates/proteins at 30 µg per lane.

    Secondary
    HRP Anti-Rabbit at 1/20000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 168 kDa
    Observed band size : 260 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 90 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 15 minutes
  • ICC/IF image of ab88491 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab88491, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-Slit1 antibody (ab88491) 使用論文

ab88491 has not yet been referenced specifically in any publications.

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Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Embryonic spinal cord)
Specification Embryonic spinal cord
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
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Mr. Carl Hobbs

Verified customer

投稿 Mar 02 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"