製品の概要
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製品名Anti-Slit1 antibody
Slit1 一次抗体 製品一覧 -
製品の詳細Mouse polyclonal to Slit1
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由来種Mouse
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アプリケーション適用あり: WB, ELISA, IHC-P, ICC/IFmore details
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種交差性交差種: Mouse, Human
交差が予測される動物種: Rat, Chicken, Xenopus laevis
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免疫原
Synthetic peptide corresponding to Mouse Slit1 aa 497-504 conjugated to Keyhole Limpet Haemocyanin (KLH).
Sequence:CSAKEQYFIPGTEDYHLN
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ポジティブ･コントロール
- IHC-P: Human Kidney tissue; Human Pancreas tissue
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特記事項Avoid repeated freezing and thawing.
製品の特性
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製品の状態Liquid
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保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term.
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バッファーpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 0.88% Sodium chloride, 0.27% Potassium phosphate -
Concentration information loading... -
精製度Immunogen affinity purified
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ポリ/モノポリクローナル
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アイソタイプIgG
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研究分野
関連製品
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Compatible Secondaries
アプリケーション
Our Abpromise guarantee covers the use of ab115892 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | 1/500 - 1/2000. Predicted molecular weight: 167 kDa. | |
| ELISA | 1/10000 - 1/50000. | |
| IHC-P | Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
| ICC/IF | Use at an assay dependent dilution. |
ターゲット情報
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機能Thought to act as molecular guidance cue in cellular migration, and function appears to be mediated by interaction with roundabout homolog receptors. During neural development involved in axonal navigation at the ventral midline of the neural tube and projection of axons to different regions (By similarity). SLIT1 and SLIT2 together seem to be essential for midline guidance in the forebrain by acting as repulsive signal preventing inappropriate midline crossing by axons projecting from the olfactory bulb.
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組織特異性Predominantly expressed in adult forebrain. Expressed in fetal brain, lung and kidney.
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配列類似性Contains 1 CTCK (C-terminal cystine knot-like) domain.
Contains 9 EGF-like domains.
Contains 1 laminin G-like domain.
Contains 20 LRR (leucine-rich) repeats.
Contains 4 LRRCT domains.
Contains 4 LRRNT domains. -
細胞内局在Secreted.
- Information by UniProt
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参照データベース
- Entrez Gene: 395293 Chicken
- Entrez Gene: 6585 Human
- Entrez Gene: 20562 Mouse
- Entrez Gene: 65047 Rat
- Entrez Gene: 380270 Xenopus laevis
- Omim: 603742 Human
- SwissProt: O75093 Human
- SwissProt: Q80TR4 Mouse
see all -
別名
- KIAA0813 antibody
- MEGF 4 antibody
- MEGF4 antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Slit1 antibody (ab115892)ab115892, at 5 ug/ml, staining Slit1 in formalin fixed, paraffin embedded Human kidney tissue by Immunohistochemistry. Developed using Goat anti rabbit biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Slit1 antibody (ab115892)ab115892, at 5 ug/ml, staining Slit1 in formalin fixed, paraffin embedded Human pancreas tissue by Immunohistochemistry. Developed using Goat anti rabbit biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen.
プロトコール
参考文献
ab115892 has not yet been referenced specifically in any publications.
レビューと Q&A
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"