Anti-Slingshot homolog 1 抗体 (ab107799)

製品の概要

  • 製品名Anti-Slingshot homolog 1 antibody
    Slingshot homolog 1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Slingshot homolog 1
  • アプリケーション適用あり: WBmore details
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Rat
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 450 - 550 of Mouse Slingshot homolog 1.

  • ポジティブ・コントロール
    • This antibody gave a positive signal in both Mouse Brain and Heart tissue lysates as well as A431 and HepG2 whole cell lysates.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファーpH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS
    Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab107799 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 150 kDa (predicted molecular weight: 115 kDa).

ターゲット情報

  • 機能Protein phosphatase which regulates actin filament dynamics. Dephosphorylates and activates the actin binding/depolymerizing factor cofilin, which subsequently binds to actin filaments and stimulates their disassembly. Inhibitory phosphorylation of cofilin is mediated by LIMK1, which may also be dephosphorylated and inactivated by this protein.
  • 配列類似性Belongs to the protein-tyrosine phosphatase family.
    Contains 1 tyrosine-protein phosphatase domain.
  • 翻訳後修飾Phosphorylated. Inhibitory phosphorylation by PAK4 promotes binding to YWHAZ. Phosphorylation at Ser-978 is decreased by stimuli which promote actin reorganization and lamellipodia formation. Can be dephosphorylated and activated by PPP3CA/calcineurin A. Phosphorylation decreases immediately prior to telophase.
  • 細胞内局在Cytoplasm > cytoskeleton. Cell projection > lamellipodium. Cleavage furrow. Midbody. Also recruited to actin rich membrane protrusions such as lamellipodia, which may allow local control of actin dynamics at sites of cell locomotion. Also localized to the cleavage furrow and the midbody during cytokinesis.
  • Information by UniProt
  • 参照データベース
  • 別名
    • AW551225 antibody
    • Coro1c antibody
    • coronin, actin binding protein 1C antibody
    • Gm1394 antibody
    • Gm1395 antibody
    • hSSH-1L antibody
    • mSSH 1L antibody
    • OTTMUSP00000031415 antibody
    • Protein phosphatase Slingshot homolog 1 antibody
    • Slingshot 1 antibody
    • slingshot 1L antibody
    • slingshot homolog 1 antibody
    • slingshot like 1 antibody
    • Slingshot1 antibody
    • SSH 1 antibody
    • SSH 1L antibody
    • SSH-1L antibody
    • SSH-like protein 1 antibody
    • Ssh1 antibody
    • SSH1_HUMAN antibody
    see all

Anti-Slingshot homolog 1 antibody 画像

  • All lanes : Anti-Slingshot homolog 1 antibody (ab107799) at 1 µg/ml

    Lane 1 : Brain (Mouse) Tissue Lysate
    Lane 2 : Heart (Mouse) Tissue Lysate
    Lane 3 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 4 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 115 kDa
    Observed band size : 150 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 56 kDa,80 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 20 minutes

Anti-Slingshot homolog 1 antibody (ab107799) 使用論文

ab107799 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Sample Mouse Tissue lysate - whole (brain tissue)
Gel Running Conditions Reduced Denaturing (12)
Loading amount 50 µg
Specification brain tissue
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Abcam user community

Verified customer

投稿 Sep 30 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Tissue lysate - whole (brain tissue)
Gel Running Conditions Reduced Denaturing (12)
Loading amount 50 µg
Specification brain tissue
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Sep 23 2016

Application Western blot
Sample Mouse Tissue lysate - whole (Brain cortex)
Gel Running Conditions Reduced Denaturing (12)
Loading amount 50 µg
Specification Brain cortex
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Jul 22 2015

Application Western blot
Sample Human Tissue lysate - whole (Brain cortex)
Gel Running Conditions Reduced Denaturing (12)
Loading amount 50 µg
Specification Brain cortex
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Jul 22 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"