Anti-SLFN5 抗体 (ab121537)
Key features and details
- Rabbit polyclonal to SLFN5
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-SLFN5 antibody -
製品の詳細
Rabbit polyclonal to SLFN5 -
由来種
Rabbit -
アプリケーション
適用あり: ICC/IF, IHC-P, WBmore details -
種交差性
交差種: Human -
免疫原
antigen sequence, corresponding to internal sequence amino acids 70-210 of Human SLFN5.
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ポジティブ・コントロール
- ICC/IF: A-431 human cell line IHC: Human lymph node, Fallopian tube and endometrium tissue Wb: SK-MEL-30 and Caco-2 cell lines
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab121537の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
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IHC-P |
1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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WB |
Use a concentration of 0.04 - 0.4 µg/ml.
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特記事項 |
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ICC/IF
Use a concentration of 0.25 - 2 µg/ml. |
IHC-P
1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
Use a concentration of 0.04 - 0.4 µg/ml. |
ターゲット情報
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機能
May have a role in hematopoeitic cell differentiation. -
配列類似性
Belongs to the Schlafen family. - Information by UniProt
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参照データベース
- Entrez Gene: 162394 Human
- SwissProt: Q08AF3 Human
- Unigene: 709347 Human
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別名
- AI956840 antibody
- RP23-392I13.5 antibody
- Schlafen 5 antibody
see all
画像
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ab121537 at 1/725 dilution staining SLFN5 in paraffin-embedded Human fallopian tube by Immunohistochemistry.
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ab121537 at 1/725 dilution staining SLFN5 in paraffin-embedded Human endometrium by Immunohistochemistry.
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ab121537 at 1/725 dilution staining SLFN5 in paraffin-embedded Human lymph node by Immunohistochemistry.
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Immunofluorescent staining of Human cell line A-431 shows positivity in nuclei but not nucleoli. Recommended concentration of ab121537 1-4 µg/ml. Cells treated with PFA/Triton X-100.
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All lanes : Anti-SLFN5 antibody (ab121537) at 0.4 µg/ml
Lane 1 : SK-MEL-30 cell lysate
Lane 2 : Caco-2 cell lysateLoading control: Anti-HDAC1
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (3)
ab121537 は 3 報の論文で使用されています。
- Martinez RS et al. SLFN5 Regulates LAT1-Mediated mTOR Activation in Castration-Resistant Prostate Cancer. Cancer Res 81:3664-3678 (2021). PubMed: 33985973
- Companioni Nápoles O et al. SCHLAFEN 5 expression correlates with intestinal metaplasia that progresses to gastric cancer. J Gastroenterol 52:39-49 (2017). PubMed: 27032393
- Ding L et al. Schlafen 4-expressing myeloid-derived suppressor cells are induced during murine gastric metaplasia. J Clin Invest 126:2867-80 (2016). PubMed: 27427984