Anti-SLD5 抗体 (ab101346)
Key features and details
- Rabbit polyclonal to SLD5
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
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製品名
Anti-SLD5 antibody
SLD5 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to SLD5 -
由来種
Rabbit -
アプリケーション
適用あり: WB, ICC/IF, IHC-Pmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Rat, Cow -
免疫原
Recombinant fragment, corresponding to amino acids 1-198 of Human SLD5
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ポジティブ・コントロール
- 293T, A431, Jurkat cell lines
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab101346の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/1000 - 1/10000. Predicted molecular weight: 26 kDa.
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ICC/IF | (1) |
1/100 - 1/500.
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IHC-P |
1/100 - 1/500. Suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH 6.0) or Tris-EDTA buffer (pH 8.0).
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特記事項 |
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WB
1/1000 - 1/10000. Predicted molecular weight: 26 kDa. |
ICC/IF
1/100 - 1/500. |
IHC-P
1/100 - 1/500. Suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH 6.0) or Tris-EDTA buffer (pH 8.0). |
ターゲット情報
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機能
The GINS complex plays an essential role in the initiation of DNA replication, and progression of DNA replication forks. GINS4 is important for GINS complex assembly. GINS complex seems to bind preferentially to single-stranded DNA. -
配列類似性
Belongs to the GINS4/SLD5 family. -
細胞内局在
Cytoplasm. Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 84296 Human
- Entrez Gene: 109145 Mouse
- Entrez Gene: 290842 Rat
- Omim: 610611 Human
- SwissProt: A2VE40 Cow
- SwissProt: Q9BRT9 Human
- SwissProt: Q99LZ3 Mouse
- SwissProt: Q499W2 Rat
see all -
別名
- DNA replication complex GINS protein SLD5 antibody
- GINS complex subunit 4 (Sld5 homolog) antibody
- GINS complex subunit 4 antibody
see all
画像
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Anti-SLD5 antibody (ab101346) at 1/1000 dilution + 293T whole cell lysate at 30 µg
Predicted band size: 26 kDa
12% SDS PAGE -
Anti-SLD5 antibody (ab101346) at 1/10000 dilution + Mouse heart at 50 µg
Predicted band size: 26 kDa -
ab101346 at 1/500 staining SLD5 in paraffin-embedded human gastric cancer.
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ab101346 at 1/200 staining paraformaldehyde-fixed A431 in IF. Bottom image costained with Hoechst 33342.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (3)
ab101346 は 3 報の論文で使用されています。
- Zhang Z et al. Overexpression of GINS4 Is Associated With Tumor Progression and Poor Survival in Hepatocellular Carcinoma. Front Oncol 11:654185 (2021). PubMed: 33842367
- Kaur M et al. Sld5 Ensures Centrosomal Resistance to Congression Forces by Preserving Centriolar Satellites. Mol Cell Biol 38:N/A (2018). PubMed: 29061732
- Kimura T et al. Induced expression of GINS complex is an essential step for reactivation of quiescent stem-like tumor cells within the peri-necrotic niche in human glioblastoma. J Cancer Res Clin Oncol N/A:N/A (2018). PubMed: 30465075