製品の概要

  • 製品名
    Anti-SLC5A8 antibody
  • 製品の詳細
    Rabbit polyclonal to SLC5A8
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Rat, Rabbit
  • 免疫原

    Synthetic peptide corresponding to a region within internal amino acids 431-480 of human SLC5A8 (NP_666018).

  • ポジティブ・コントロール
    • OVCAR-3 cell lysate

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab99064 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 67 kDa.Can be blocked with SLC5A8 peptide (ab117479). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

ターゲット情報

  • 機能
    Acts as an electrogenic sodium (Na(+)) and chloride (Cl-)-dependent sodium-coupled solute transporter, including transport of monocarboxylates (short-chain fatty acids including L-lactate, D-lactate, pyruvate, acetate, propionate, valerate and butyrate), lactate, mocarboxylate drugs (nicotinate, benzoate, salicylate and 5-aminosalicylate) and ketone bodies (beta-D-hydroxybutyrate, acetoacetate and alpha-ketoisocaproate), with a Na(+):substrate stoichiometry of between 4:1 and 2:1. Catalyzes passive carrier mediated diffusion of iodide. Mediates iodide transport from the thyrocyte into the colloid lumen through the apical membrane. May be responsible for the absorption of D-lactate and monocarboxylate drugs from the intestinal tract. Acts as a tumor suppressor, suppressing colony formation in colon cancer, prostate cancer and glioma cell lines. May play a critical role in the entry of L-lactate and ketone bodies into neurons by a process driven by an electrochemical Na(+) gradient and hence contribute to the maintenance of the energy status and function of neurons.
  • 組織特異性
    Expressed in normal thyroid, localized at the apical pole of thyroid cells facing the colloid lumen, but expression profoundly decreased in thyroid carcinomas. Expressed in normal colon but absent in colon aberrant crypt foci and colon cancers. Present in normal kidney cortex, brain, prostate, gastric mucosa and breast tissue but was significantly down-regulated in primary gliomas, gastric cancer, prostate tumors and breast tumors.
  • 配列類似性
    Belongs to the sodium:solute symporter (SSF) (TC 2.A.21) family.
  • 細胞内局在
    Apical cell membrane. Expressed at the apical membrane of normal tall thyrocytes and of colonic epithelial cells.
  • Information by UniProt
  • 参照データベース
  • 別名
    • AIT antibody
    • Apical iodide transporter antibody
    • Electrogenic sodium monocarboxylate cotransporter antibody
    • MGC125354 antibody
    • SC5A8_HUMAN antibody
    • SLC5A8 antibody
    • SMCT antibody
    • SMCT1 antibody
    • sodium coupled monocarboxylate transporter 1 antibody
    • Sodium iodide related cotransporter antibody
    • Sodium iodide-related cotransporter antibody
    • Sodium-coupled monocarboxylate transporter 1 antibody
    • solute carrier family 5 iodide transporter member 8 antibody
    • Solute carrier family 5 member 8 antibody
    see all

Anti-SLC5A8 antibody 画像

  • Anti-SLC5A8 antibody (ab99064) at 1 µg/ml + OVCAR-3 cell lysate at 10 µg

    Predicted band size : 67 kDa

プロトコール

Anti-SLC5A8 antibody (ab99064) 使用論文

This product has been referenced in:
  • Cresci G  et al. Lactobacillus GG and tributyrin supplementation reduce antibiotic-induced intestinal injury. JPEN J Parenter Enteral Nutr 37:763-74 (2013). Read more (PubMed: 23630018) »

See 1 Publication for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Pig Tissue sections (Pancreas)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris-EDTA, pH 9.0
Permeabilization
No
Specification
Pancreas
Blocking step
Serum as blocking agent for 20 minute(s) · Concentration: 10% · Temperature: 22°C
Fixative
Paraformaldehyde
Username

Kaiyuan Yang

Verified customer

投稿 Apr 28 2017

Application
Western blot
Sample
Rat Cell lysate - other (Kidney Cortex)
Gel Running Conditions
Reduced Denaturing (10% gel)
Loading amount
15 µg
Specification
Kidney Cortex
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

投稿 May 18 2016

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products. I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused...

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Thank you for providing me with these additional details and for your cooperation. The details provided enable us to closely monitor the quality of our products. In regard to the choice of positive control for this product. I have spoken with the l...

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Thank you for contacting us.   We value any additions to our abreview program whether the result are good or bad and I encourage you to please submit an abreview so that other researchers may gain insight from your experiments. By placing an abreview y...

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Yes, the catalogue number for the peptide is ab117479 and the datasheet can be found at the following web address: http://www.abcam.com/SLC5A8-peptide-ab117479.html.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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