製品の概要

  • 製品名Anti-SLC38A5 / SNAT5 antibody
    SLC38A5 / SNAT5 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to SLC38A5 / SNAT5
  • アプリケーション適用あり: WB, IHC-P, ICC/IFmore details
  • 種交差性
    交差種: Rat, Human
    交差が予測される動物種: Mouse
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Rat SLC38A5/ SNAT5.

    (Peptide available as ab92656.)

  • ポジティブ・コントロール
    • This antibody gave a positive signal in the following rat tissue lysates: Cortex; Hippocampus; Spinal Cord. This antibody gave a positive result in IHC in the following FFPE tissue: Rat normal pancreas.

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab72717 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 52 kDa (predicted molecular weight: 52 kDa).
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use at an assay dependent concentration.

ターゲット情報

  • 機能Functions as a sodium-dependent amino acid transporter which countertransport protons. Mediates the saturable, pH-sensitive, and electrogenic cotransport of several neutral amino acids including glycine, asparagine, alanine, serine, glutamine and histidine with sodium.
  • 組織特異性Predominantly expressed in stomach, brain, liver, lung and intestinal tract.
  • 配列類似性Belongs to the amino acid/polyamine transporter 2 family.
  • 細胞内局在Cell membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Amino acid transporter system N2 antibody
    • JM 24 antibody
    • JM24 antibody
    • pp7194 antibody
    • S38A5 antibody
    • S38A5_HUMAN antibody
    • Slc38a5 antibody
    • SLC8 A5 antibody
    • SLC8A 5 antibody
    • SN 2 antibody
    • SN2 antibody
    • SNAT 5 antibody
    • SNAT5 antibody
    • Sodium coupled neutral amino acid transporter 5 antibody
    • Sodium coupled neutral amino acid transporter5 antibody
    • Sodium-coupled neutral amino acid transporter 5 antibody
    • Solute carrier family 38 member 5 antibody
    • Solute carrier family 38 member5 antibody
    • System N transporter 2 antibody
    • System N transporter2 antibody
    see all

Anti-SLC38A5 / SNAT5 antibody 画像

  • IHC image of SLC38A5 / SNAT5 staining in Rat normal pancreas formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab72717, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

     

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • All lanes : Anti-SLC38A5 / SNAT5 antibody (ab72717) at 1 µg/ml

    Lane 1 : Cortex (Rat) Tissue Lysate
    Lane 2 : Hippocampus (Rat) Tissue Lysate
    Lane 3 : Spinal Cord (Rat) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 52 kDa
    Observed band size : 52 kDa
    Additional bands at : 28 kDa,30 kDa,40 kDa. We are unsure as to the identity of these extra bands.
  • ICC/IF image of ab72717 stained PC12 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab72717, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-SLC38A5 / SNAT5 antibody (ab72717) 使用論文

This product has been referenced in:
  • Day PE  et al. Partitioning of glutamine synthesised by the isolated perfused human placenta between the maternal and fetal circulations. Placenta 34:1223-31 (2013). WB ; Human . Read more (PubMed: 24183194) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"