製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to SLC38A1
  • アプリケーション
    適用あり: WB, IHC-Pmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Rat, Horse, Guinea pig, Cow, Cat, Dog
  • 免疫原

    A region within synthetic peptide: YLGYTSGFSL SCMVFFLIVV IYKKFQIPCI VPELNSTISA NSTNADTCTP, corresponding to internal sequence amino acids 217-266 of Human SLC38A1

  • ポジティブ・コントロール
    • HepG2 cell lysate; human kidney tissue

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab60145 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1.25 µg/ml. Detects a band of approximately 48 kDa (predicted molecular weight: 59 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 4 - 8 µg/ml.

ターゲット情報

  • 機能
    Functions as a sodium-dependent amino acid transporter. Mediates the saturable, pH-sensitive and electrogenic cotransport of glutamine and sodium ions with a stoichiometry of 1:1. May also transport small zwitterionic and aliphatic amino acids with a lower affinity. May supply glutamatergic and GABAergic neurons with glutamine which is required for the synthesis of the neurotransmitters glutamate and GABA.
  • 組織特異性
    Expressed in the cerebral cortex by pyramidal and GABAergic neurons, astrocytes and other non-neuronal cells (at protein level). Expressed in placenta, heart, lung, skeletal muscle, spleen, stomach and testis.
  • 配列類似性
    Belongs to the amino acid/polyamine transporter 2 family.
  • 細胞内局在
    Cell membrane. Restricted to the somatodendritic compartment of neurons. Found in the cellular processes of neurons in the developing brain (By similarity).
  • Information by UniProt
  • 参照データベース
  • 別名
    • Amino acid transporter A1 antibody
    • Amino acid transporter system A1 antibody
    • ATA 1 antibody
    • ATA1 antibody
    • N-system amino acid transporter 2 antibody
    • NAT 2 antibody
    • NAT2 antibody
    • S38A1_HUMAN antibody
    • SAT 1 antibody
    • SAT1 antibody
    • SLC38A1 antibody
    • SNAT 1 antibody
    • SNAT1 antibody
    • Sodium-coupled neutral amino acid transporter 1 antibody
    • Solute carrier family 38 member 1 antibody
    • System A amino acid transporter 1 antibody
    • System N amino acid transporter 1 antibody
    see all

画像

  • Anti-SLC38A1 antibody (ab60145) at 1.25 µg/ml + HepG2 cell lysate at 10 µg

    Secondary
    HRP-conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 59 kDa
    Observed band size : 48 kDa (why is the actual band size different from the predicted?)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling SLC38A1 with ab60145 at 4-8µg/ml. Arrows indicate positively labelled epithelial cells of the renal tubule. Magnification: 400X.

参考文献

This product has been referenced in:
  • Dikalova A  et al. Sodium-coupled neutral amino acid transporter 1 (SNAT1) modulates L-citrulline transport and nitric oxide (NO) signaling in piglet pulmonary arterial endothelial cells. PLoS One 9:e85730 (2014). WB ; Pig . Read more (PubMed: 24454923) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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