製品の概要

  • 製品名Anti-SLC37A4 antibody
    SLC37A4 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to SLC37A4
  • アプリケーション適用あり: ICC/IF, WB, ELISAmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog, Pig
  • 免疫原

    Synthetic peptide: PSLVEEIPLD KDDLGFITSS QSAAYAISKF VSGVLSDQMS ARWLFSSGLL , corresponding to N terminal amino acids 35-84 of Human SLC37A4 (NP_001458)

  • ポジティブ・コントロール
    • Fetal brain lysate.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab80463 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use at an assay dependent concentration.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 46 kDa (predicted molecular weight: 46 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1/312500.

ターゲット情報

  • 機能Transports glucose-6-phosphate from the cytoplasm to the lumen of the endoplasmic reticulum. Forms with glucose-6-phosphatase the complex responsible for glucose production through glycogenolysis and gluconeogenesis. Hence, it plays a central role in homeostatic regulation of blood glucose levels.
  • 組織特異性Mostly expressed in liver and kidney.
  • 関連疾患Defects in SLC37A4 are the cause of glycogen storage disease type 1B (GSD1B) [MIM:232220]. GSD1B is a metabolic disorder characterized by impairment of terminal steps of glycogenolysis and gluconeogenesis. GSD1 patients manifest a wide range of clinical symptoms and biochemical abnormalities, including hypoglycemia, severe hepatomegaly due to excessive accumulation of glycogen, kidney enlargement, growth retardation, lactic acidemia, hyperlipidemia, and hyperuricemia. GSD1B patients also present a tendency towards infections associated with neutropenia, relapsing aphthous gingivostomatitis, and inflammatory bowel disease.
    Defects in SLC37A4 are the cause of glycogen storage disease type 1C (GSD1C) [MIM:232240].
    Defects in SLC37A4 are the cause of glycogen storage disease type 1D (GSD1D) [MIM:232240].
  • 配列類似性Belongs to the major facilitator superfamily. Organophosphate:Pi antiporter (OPA) (TC 2.A.1.4) family.
  • 細胞内局在Endoplasmic reticulum membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • G6PT1 antibody
    • G6PT1_HUMAN antibody
    • G6PT2 antibody
    • G6PT3 antibody
    • Glucose-5-phosphate transporter antibody
    • Glucose-6-phosphatase, transport (glucose) protein 3 antibody
    • Glucose-6-phosphatase, transport (glucose-6-phosphate) protein 1 antibody
    • Glucose-6-phosphatase, transport (phosphate/pyrophosphate) protein 2; antibody
    • Glucose-6-phosphate translocase antibody
    • Glucose-6-phosphate transporter 1 antibody
    • GSD1b antibody
    • GSD1c antibody
    • GSD1d antibody
    • MGC15729 antibody
    • Microsomal glucose-6-phosphate transporter antibody
    • PRO0685 antibody
    • SLC37A4 antibody
    • Solute carrier family 37 (glucose-6-phosphate transporter), member 4 antibody
    • Solute carrier family 37 member 4 antibody
    • Transformation-related gene 19 protein antibody
    • TRG-19 antibody
    • TRG19 antibody
    see all

Anti-SLC37A4 antibody 画像

  • ab80463 staining SLC37A4 in Mouse bone marrow cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with formaldehyde, permeabilized with Triton X-100 0.1% and blocked with 5% serum for 2 hours at 21°C. Samples were incubated with primary antibody (1/100 in PBS + 2% BSA) for 12 hours at 4°C. An Alexa Fluor®488-conjugated Goat anti-rabbit IgG polyclonal  (1/500)was used as the secondary antibody.

    See Abreview

  • Anti-SLC37A4 antibody (ab80463) at 1 µg/ml + Fetal brain lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 46 kDa
    Observed band size : 46 kDa
    Additional bands at : 25 kDa. We are unsure as to the identity of these extra bands.

Anti-SLC37A4 antibody (ab80463) 使用論文

ab80463 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (White blood cells)
Specification White blood cells
Fixative Formaldehyde
Blocking step Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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投稿 Apr 09 2013

Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (Bone marrow cells)
Specification Bone marrow cells
Fixative Formaldehyde
Permeabilization Yes - 0.1% TritonX-100
Blocking step Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

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Verified customer

投稿 Apr 09 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"