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Affinity purified using the PrEST antigen as affinity ligand.
Our Abpromise guarantee covers the use of ab121540 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
|IHC-P||1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
ab121540 at 1/500 dilution staining SLC36A4 in paraffin-embedded Human hippocampus tissue by Immunohistochemistry.
ab121540 has not yet been referenced specifically in any publications.