製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to SLC2A4RG
  • アプリケーション
    適用あり: ICC/IF, WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Horse, Chimpanzee, Macaque monkey, Gorilla
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 200 - 300 of Human SLC2A4RG.

  • ポジティブ・コントロール
    • This antibody gave a positive signal in Human Skeletal Muscle tissue lysate. This antibody gave a positive result in IF in the following Methanol fixed cell line: MCF-7

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab86756 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 41 kDa (predicted molecular weight: 41 kDa).

ターゲット情報

  • 機能
    Transcription factor involved in SLC2A4 and HD gene transactivation. Binds to the consensus sequence 5'-GCCGGCG-3'.
  • 組織特異性
    According to PubMed:14630949, expressed in heart, skeletal muscle, liver, kidney and pancreas; undetectable in lung, placenta or brain. According to PubMed:14625278, ubiquitously expressed, with lowest expression in brain and ileum.
  • 配列類似性
    Contains 1 C2H2-type zinc finger.
  • 細胞内局在
    Cytoplasm. Nucleus. Shuttles between the cytoplasm and the nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • GEF antibody
    • GLUT4 enhancer factor antibody
    • HDBP 1 antibody
    • HDBP-1 antibody
    • HDBP1 antibody
    • Huntington disease gene regulatory region binding protein 1 antibody
    • Huntington disease gene regulatory region-binding protein 1 antibody
    • S2A4R_HUMAN antibody
    • Si 1 2 19 antibody
    • Si 1 2 antibody
    • SLC2A4 regulator antibody
    • SLC2A4RG antibody
    • Zinc finger protein 2 5 Glut4 enhancer factor antibody
    see all

画像

  • Anti-SLC2A4RG antibody (ab86756) at 1 µg/ml + Human skeletal muscle tissue lysate - total protein (ab29330) at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 41 kDa
    Observed band size : 41 kDa
    Additional bands at : 24 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 4 minutes
  • ICC/IF image of ab86756 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab86756 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in formaldehyde fixed (4%, 10min) HeLa, Hek293, HepG2, and MCF-7 cell types, and in Methanol fixed (100%, 5min) Hek293 cell types.

参考文献

ab86756 has not yet been referenced specifically in any publications.

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