製品の概要

  • 製品名Anti-SLC27A6 antibody
    SLC27A6 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to SLC27A6
  • アプリケーション適用あり: WB, IHC-P, ICC/IFmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Pig, Drosophila melanogaster, Zebrafish
  • 免疫原

    Synthetic peptide corresponding to a region within the middle amino acids 215-264 (KSTCLYIFTSGTTGLPKAAVISQLQVLRGSAVLWAFGCTAHDIVYITLP L) of Human SLC27A6 (NP_001017372)

  • ポジティブ・コントロール
    • MCF7 membrane lysate

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab84183 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 70 kDa (predicted molecular weight: 70 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P 1/100.
ICC/IF Use a concentration of 5 µg/ml.

ターゲット情報

  • 機能Involved in translocation of long-chain fatty acids (LFCA) across the plasma membrane. Thought to function as the predominant fatty acid protein transporter in heart.
  • 組織特異性Strongly expressed in heart and localizes to cardiac myocytes. Expressed at moderate levels in placenta, testis, and adrenal glands. Expressed at very low levels in kidney, bladder and uterus.
  • 配列類似性Belongs to the ATP-dependent AMP-binding enzyme family.
  • 細胞内局在Membrane. Cell membrane > sarcolemma. In heart is exclusively located on the sarcolemma in areas juxtaposed with small blood vessels where it colocalizes CD36.
  • Information by UniProt
  • 参照データベース
  • 別名
    • ACSVL2 antibody
    • FACVL2 antibody
    • FATP 6 antibody
    • FATP-6 antibody
    • FATP1 antibody
    • FATP6 antibody
    • Fatty acid coenzyme A ligase, very long chain 2 antibody
    • Fatty acid transport protein 6 antibody
    • Fatty-acid-coenzyme A ligase antibody
    • hVLCS H1 antibody
    • hVLCS-H1 antibody
    • Long-chain fatty acid transport protein 6 antibody
    • S27A6_HUMAN antibody
    • SLC27A6 antibody
    • solute carrier family 27 fatty acid transporter member 6 antibody
    • Solute carrier family 27 member 6 antibody
    • Very long chain acyl CoA synthetase homolog 1 antibody
    • very long-chain 2 antibody
    • Very long-chain acyl-CoA synthetase homolog 1 antibody
    • VLCS H1 antibody
    • VLCSH1 antibody
    see all

Anti-SLC27A6 antibody 画像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human pineal tissue labelling SLC27A6 with ab84183 at 1/100. A Cy3-conjugated donkey anti-rabbit IgG (1/200) was used as the secondary antibody. Positive staining shown in the membrane and cytoplasm of cell bodies of pinealocytes and their processes. Magnification: 20X. Exposure time: 0.5 - 2.0 seconds. Left - DAPI. Middle - SLC27A6. Right - Merge.
  • Anti-SLC27A6 antibody (ab84183) at 1 µg/ml + MCF7 membrane lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 70 kDa
    Observed band size : 70 kDa
  • ICC/IF image of ab84183 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab84183, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-SLC27A6 antibody (ab84183) 使用論文

This product has been referenced in:
  • Gaccioli F  et al. Maternal overweight induced by a diet with high content of saturated fat activates placental mTOR and eIF2 alpha signaling and increases fetal growth in rats. Biol Reprod 89:96 (2013). Rat . Read more (PubMed: 24006279) »

See 1 Publication for this product

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