製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to SLC25A25
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Pig, Zebrafish
  • 免疫原

    Synthetic peptide derived from Human SLC25A25

  • ポジティブ・コントロール
    • HepG2 cell lysate.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab62267 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 0.25 µg/ml. Detects a band of approximately 52 kDa (predicted molecular weight: 56 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

ターゲット情報

  • 機能
    Calcium-dependent mitochondrial solute carrier. Mitochondrial solute carriers shuttle metabolites, nucleotides, and cofactors through the mitochondrial inner membrane. May act as a ATP-Mg/Pi exchanger that mediates the transport of Mg-ATP in exchange for phosphate, catalyzing the net uptake or efflux of adenine nucleotides into or from the mitochondria.
  • 組織特異性
    Present in various cell lines (at protein level). Widely expressed. Expressed in fetal and adult liver, skeletal muscle, testis, ovary, hippocampus and caudate nucleus. Isoform 1 is present in all tissues tested. Isoform 2 expression is restricted to kidney and lung.
  • 配列類似性
    Belongs to the mitochondrial carrier (TC 2.A.29) family.
    Contains 3 EF-hand domains.
    Contains 3 Solcar repeats.
  • 細胞内局在
    Mitochondrion inner membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Calcium-binding mitochondrial carrier protein SCaMC-2 antibody
    • KIAA1896 antibody
    • MCSC antibody
    • MGC105138 antibody
    • MGC119514 antibody
    • MGC119515 antibody
    • MGC119516 antibody
    • MGC119517 antibody
    • Mitochondrial ATP Mg/Pi carrier antibody
    • Mitochondrial ATP-Mg/Pi carrier protein 3 antibody
    • Mitochondrial Ca(2+)-dependent solute carrier protein 3 antibody
    • Mitochondrial Ca2+ dependent solute carrier antibody
    • Mitochondrial calcium-dependent solute carrier antibody
    • PCSCL antibody
    • RP11 395P17.4 antibody
    • SCAMC 2 antibody
    • SCAMC2 antibody
    • SCMC2_HUMAN antibody
    • Short calcium binding mitochondrial carrier 2 antibody
    • SLC25A25 antibody
    • Small calcium binding mitochondrial carrier 2 antibody
    • Small calcium-binding mitochondrial carrier protein 2 antibody
    • Solute carrier family 25 (mitochondrial carrier phosphate carrier) member 25 antibody
    • Solute carrier family 25 member 25 antibody
    • solute carrier family 25, member 25, isoform a antibody
    see all

画像

  • SLC25A25 antibody (ab62267) at 0.25µg/ml in 5% skim milk / PBS buffer + HepG2 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 56 kDa
    Observed band size : 52 kDa (why is the actual band size different from the predicted?)

プロトコール

参考文献

ab62267 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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